CycLex Cellular BrdU ELISA Kit is a non-isotopic immunoassay
used for the semi-quantitative measurement of BrdU (bromodeoxyuridine) incorporation in newly
synthesized DNA during DNA synthesis.
A rapid and convenient method for estimating S-phase cells in a population was developed which detects bromodeoxyuridine (BrdU) incorporation into DNA by means of monoclonal anti-BrdU antibodies in a cellular ELISA format. It has been shown that a precise evaluation of cell proliferation could be performed by the measurement of BrdU incorporation in newly synthesized cellular DNA. In addition, there is a good correlation between the cellular BrdU ELISA and the [3H]-thymidine incorporation assay as shown for a variety of murine and human cell systems, including mitogen- and antigen-stimulated lymphocytes and cytokine-induced proliferation of different cell lines.