Cellular Acetylation Assay Kit

Acetylation and deacetylation of nucleosomal histones play an important role in the modulation of chromatin structure, chromatin function and in the regulation of gene expression. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) are two opposing classes of enzymes, which tightly control the equilibrium of histone acetylation. An imbalance in the equilibrium of histone acetylation has been associated with carcinogenesis and cancer progression. So far, a number of structurally distinct classes of compounds have been identified as HDAC inhibitors including the short-chain fatty acids, hydroxamates, cyclic tetrapeptides and benzamides.

CycLex Histone Acetylation Kit is a cell-based ELISA formatted for conventional chromometric detection of relative levels of acetylated histones in microplate cells cultures. Adherent cells are cultured in conventional 96-well microplates, treated with agents that induce histone acetylation, such as Trichostatin A, a specific HDAC inhibitor, and are then fixed and permeabilized. Several types of histones, including histone H3 and H4, which are acetylated at several lysine residues are detected by anti-acetylated histone/p53-K382 monoclonal antibody.

This measuring principle and kit are covered under CycLex's patents.
U.S. Patent No. 6,884,597

Cellular Histone Acetylation Assay Kit