| Data |
Western Blotting

Immunoprecipitation

|
| Clonality |
Monoclonal
|
Clone |
3E8 |
|
Isotype
(Immunized Animal)
|
Mouse IgG2bκ
|
| Applications |
- WB
- 1 µg/mL
 - IP
- 2.5 µg/300 µL of cell extract from 3x106 cells
- IC
- 0.5 μg/mL
|
| Immunogen
(Antigen) |
Recombinant Human Atg3
|
| Reactivity
[Gene ID] |
Human[64422], Mouse[67841], Rat[171415], Hamster[100765208] |
| Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
| Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
| Alternative names |
autophagy related 3, ATG3, APG3, APG3L, PC3-96, APG3-LIKE
|
| Related products |
PM050 Anti-Atg5 pAb M153-3 Anti-Atg5 mAb PM039 Anti-Atg7 (Human) pAb PD042 Anti-Atg9A pAb M154-3 Anti-Atg12 (Human) mAb PM040 Anti-Atg16L pAb M150-3 Anti-Atg16L mAb PM036 Anti-LC3 pAb M152-3 Anti-LC3 mAb PM037 Anti-GABARAP pAb M135-3 Anti-GABARAP mAb PM038 Anti-GATE-16 pAb M134-3 Anti-Atg4B mAb PM072 Anti-VMP1 pAb PM090 Anti-Atg8 (Filamentous fungi) pAb M152-3MS Anti-LC3 mAb for My select sampler set M186-3 Anti-LC3 mAb PD014 Anti-LC3 pAb
|
| Citations |
Western Blotting - Otomo C et al. Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy. Nat Struct Mol Biol. 20, 59-66 (2013)(PMID:24191030)
- Metlagel Z et al. Structural basis of ATG3 recognition by the autophagic ubiquitin-like protein ATG12. PNAS. 110, 18844-9 (2013)(PMID:24191030)
- Choi J et al. The parasitophorous vacuole membrane of Toxoplasma gondii is targeted for disruption by ubiquitin-like conjugation systems of autophagy. Immunity. 40, 924-35 (2014)(PMID:24931121)
- Yamashita SI et al. Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. J Cell Biol. 215, 649-665 (2016)(PMID:27903607)
- Allavena G et al. Suppressed translation and ULK1 degradation as potential mechanisms of autophagy limitation under prolonged starvation. Autophagy 12, 2085–2097 (2016)(PMID:27629431)
|
| Product category |
- Research area
- Autophagy
Cellular stress
|