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HOME > Product search results > Code No. PM038 Anti-GATE-16 pAb

Code No. PM038

Anti-GATE-16 pAb

Availability (in Japan)

10 or more

(In Japan at 17:00,
Oct 6, 2022 in JST)


100 µL

  • Western Blotting

  • Western Blotting

Clonality Polyclonal Clone Polyclonal
Isotype (Immunized Animal) Rabbit Ig (aff.)
reported  (PMID: 21684337
Immunogen (Antigen) Carrier protein conjugated GATE-16 N-terminal synthetic peptide
Reactivity [Gene ID]

Human[11345], Mouse[93739], Rat[64670], Hamster[100767407]

Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names GABARAPL2, GABA(A) receptor-associated protein-like 2, ATG8, GEF2, ATG8C, GEF-2, GATE16
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. LC3, GABARAP (Gamma-aminobutyric acid type A receptor-associated protein), and GATE-16 (Golgi-associated ATPase enhancer of 16 kDa) have been identified as a homologue of yeast Atg8. These homologues have been characterized as modifiers in reactions mediated by hAtg7 (an E1-like enzyme) and hAtg3 (an E2-like enzyme) as in yeast Atg8 lipidation. These homologues also generate form II, which are recovered in membrane fractions. Generation of the form II correlates with autophagosome association. These results suggest that all mammalian Atg8 homologues receive a common modification to associate with autophagosomal membrane as the form II.
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Notes This antibody does not detect LC3 and GABARAP.

Western Blotting

  1. Fujita N et al. An Atg4B mutant hampers the lipidation of LC3 paralogues and causes defects in autophagosome closure. Mol Biol Cell 19, 4651-9 (2008)(PMID:18768752)
  2. Tanji K et al. Alteration of autophagosomal proteins (LC3, GABARAP and GATE-16) in Lewy body disease. Neurobiol Dis. 43, 690-7 (2011)(PMID:21684337)
  3. Niso-Santano M et al. Unsaturated fatty acids induce non-canonical autophagy. EMBO J. 34, 1025-41 (2015)(PMID:25586377)
  4. Polletta L et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy 11, 253-70 (2015)(PMID:25700560)
  5. Skytte Rasmussen M et al. ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8. Autophagy 13, 834-853 (2017)(PMID:28287329)
  6. Winer H et al. Autophagy differentially regulates TNF receptor Fn14 by distinct mammalian Atg8 proteins. Nat Commun. 9, 3744 (2018)(PMID:30218067)


  1. Tanji K et al. Alteration of autophagosomal proteins (LC3, GABARAP and GATE-16) in Lewy body disease. Neurobiol Dis. 43, 690-7 (2011)(PMID:21684337)
Product category
Research area

  • The availability is based on the information in Japan at 17:00, Oct 6, 2022 in JST.
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  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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