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HOME > Product search results > Code No. M134-3 Anti-Atg4B mAb

Code No. M134-3

Anti-Atg4B mAb

Availability (in Japan)

10 or more

(In Japan at 17:00,
Oct 6, 2022 in JST)


100 µg/100 µL

  • Western Blotting

Clonality Monoclonal Clone 9H5
Isotype (Immunized Animal) Mouse IgG1
1 µg/mL  
Immunogen (Antigen) Human full-length Atg4B (1-393 a.a.)
Reactivity [Gene ID]

Human[23192], Mouse[66615], Rat[316640], Hamster[100754964]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C Conjugate Unlabeled Manufacturer MBL
Alternative names autophagy related 4B cysteine peptidase, APG4B, AUTL1
Background Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Atg4B is a cysteine protease required for autophagy process, which cleaves the carboxyl-terminus of LC3, to produce LC3-I. LC3-I is activated by the E1-like enzyme Atg7 and forms a Atg7-LC3-I thioester. Atg7-LC3-I is transferred to Atg3 to form Atg3-LC3-I thioester. Atg3 is an E2-like enzyme that catalyzes the conjugation of LC3-I and phosphatidylethanolamine (PE) to form LC3-II. The LC3-II-PE conjugate is essential for binding tightly to autophagosomal membrane.
Related products M133-3 Anti-Atg3 mAb
PM036 Anti-LC3 pAb
M152-3 Anti-LC3 mAb
M186-3 Anti-LC3 mAb
PD014 Anti-LC3 pAb
PM039 Anti-Atg7 (Human) pAb
PM072 Anti-VMP1 pAb

Western Blotting

  1. Kang YA et al. Autophagy driven by a master regulator of hematopoiesis. Mol Cell Biol. 32, 226-39 (2012)(PMID:22025678)
  2. Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013)(PMID:24141421)
  3. Allavena G et al. Suppressed translation and ULK1 degradation as potential mechanisms of autophagy limitation under prolonged starvation. Autophagy 12, 2085–2097 (2016)(PMID:27629431)
  4. Chu J et al. ATG4B inhibitor FMK-9a induces autophagy independent on its enzyme inhibition. Arch Biochem Biophys. 644, 29-36 (2018)(PMID:29510087)
Product category
Research area

  • The availability is based on the information in Japan at 17:00, Oct 6, 2022 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.