Antibody detailed product information

Code No. M133-3 プリントPrint out
Anti-Atg3 mAb
Size 100 µg/100 µL
Availability (in Japan) 10 or more
(In Japan at 00:05, Nov 30, 2023 in JST)
Clonality Monoclonal
Clone 3E8
(Immunized Animal)
Mouse IgG2bκ
Applications WB 1 µg/mL  
IP 2.5 µg/300 µL of cell extract from 3x106 cells  
IC 0.5 μg/mL  
Recombinant Human Atg3
Reactivity [Gene ID]

Human[64422], Mouse[67841], Rat[171415], Hamster[100765208]

Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names autophagy related 3, ATG3, APG3, APG3L, PC3-96, APG3-LIKE
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Product category Research area:Autophagy Cellular stress


Western Blotting

  1. Otomo C et al. Structure of the human ATG12~ATG5 conjugate required for LC3 lipidation in autophagy. Nat Struct Mol Biol. 20, 59-66 (2013)(PMID:24191030)
  2. Metlagel Z et al. Structural basis of ATG3 recognition by the autophagic ubiquitin-like protein ATG12. PNAS. 110, 18844-9 (2013)(PMID:24191030)
  3. Choi J et al. The parasitophorous vacuole membrane of Toxoplasma gondii is targeted for disruption by ubiquitin-like conjugation systems of autophagy. Immunity. 40, 924-35 (2014)(PMID:24931121)
  4. Yamashita SI et al. Mitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagy. J Cell Biol. 215, 649-665 (2016)(PMID:27903607)
  5. Allavena G et al. Suppressed translation and ULK1 degradation as potential mechanisms of autophagy limitation under prolonged starvation. Autophagy 12, 2085–2097 (2016)(PMID:27629431)
  • The availability is based on the information in Japan at 00:05, Nov 30, 2023 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications:
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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