Data |
Western Blotting

Immunoprecipitation

RNP Immunoprecipitation

|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit Ig (aff.)
|
Applications |
- WB
- 1:1,000
 - IP
- 5 µL/250 µL of cell extract from 2.5 x 106 cells
 - RIP
- 15 µL/500 µL of cell extract from 1.5 x 107 cells
 - ChIP*
- reported. (PMID: 24165022)

|
Immunogen
(Antigen) |
KLH-conjugated synthetic peptide QNYQNSESGEKNEGSES (160-176 aa)
|
Reactivity
[Gene ID] |
Human[4904], Mouse[22608], Rat[500538], Hamster |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
Y box binding protein 1, YB1
|
Related products |
RN1001 RIP-Assay Kit RN1005 RIP-Assay Kit for microRNA RN048PW Anti-G3BP1 pAb
|
Citations |
Western Blotting - Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)
- El-Naggar AM et al. Class I HDAC inhibitors enhance YB-1 acetylation and oxidative stress to block sarcoma metastasis. EMBO Rep. 20, e48375 (2019)
(PMID:31668005)
Immunoprecipitation - Hosono Y et al. Oncogenic Role of THOR, a Conserved Cancer/Testis Long Non-coding RNA. Cell 171, 1559-1572 (2017)(PMID:29245011)
- El-Naggar AM et al. Class I HDAC inhibitors enhance YB-1 acetylation and oxidative stress to block sarcoma metastasis. EMBO Rep. 20, e48375 (2019)(PMID:31668005)
RNP Immunoprecipitation - Somasekharan SP et al. YB-1 regulates stress granule formation and tumor progression by translationally activating G3BP1. J Cell Biol. 208, 913-29 (2015)(PMID:25800057)
- Kotake Y et al. Y-box Binding Protein 1 Is Involved in Regulating the G2/M Phase of the Cell Cycle. Anticancer Res. 37, 1603-1608 (2017)(PMID:28373420)
- Kwon E et al. The RNA-binding protein YBX1 regulates epidermal progenitors at a posttranscriptional level. Nat Commun. 9, 1734 (2018)(PMID:29712925)
Other - Kotake Y et al. YB1 binds to and represses the p16 tumor suppressor gene. Genes Cell. 18, 999-1006 (2013)(PMID:24165022)
|
Product category |
- Research area
- RNA-RNP Network
Epigenetics - Brands
- RiboCluster Profiler™
|