Background |
Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively. |
Citations |
Western Blotting - Ohshima T et al. HTLV‐1 basic leucine‐zipper factor, HBZ, interacts with MafB and suppresses transcription through a Maf recognition element. J Cell Biochem. 111, 187-94 (2010)(PMID:20506502)
- Kim TS et al. The ZFHX3 (ATBF1) transcription factor induces PDGFRB, which activates ATM in the cytoplasm to protect cerebellar neurons from oxidative stress. Dis Model Mech. 3, 752-62 (2010)(PMID:20876357)
- Mukai R, Ohshima T. Dual effects of HTLV-1 bZIP factor in suppression of interferon regulatory factor 1. Biochem Biophys Res Commun. 409, 328-32 (2011)(PMID:21586271)
- Mukai R, Ohshima T. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. 33, 2317-28 (2014)(PMID:23708656)
- Zhang HH et al. Nemo-like kinase is critical for p53 stabilization and function in response to DNA damage. Cell Death Differ. 21, 1656-63 (2014)(PMID:24926618)
- Mukai R, Ohshima T. Enhanced Stabilization of MCL1 by the Human T-Cell Leukemia Virus Type 1 bZIP Factor Is Modulated by Blocking the Recruitment of Cullin 1 to the SCF Complex. Mol Cell Biol. 36, 3075-3085 (2016)(PMID:27697867)
- Kitao T et al. Legionella Manipulates Non-canonical SNARE Pairing Using a Bacterial Deubiquitinase. Cell Rep. 32, 108107 (2020)
(PMID:32905772)
Co-IP - Ohshima T et al. HTLV‐1 basic leucine‐zipper factor, HBZ, interacts with MafB and suppresses transcription through a Maf recognition element. J Cell Biochem. 111, 187-94 (2010)(PMID:20506502)
- Mukai R, Ohshima T. Dual effects of HTLV-1 bZIP factor in suppression of interferon regulatory factor 1. Biochem Biophys Res Commun. 409, 328-32 (2011)(PMID:21586271)
- Mukai R, Ohshima T. Enhanced Stabilization of MCL1 by the Human T-Cell Leukemia Virus Type 1 bZIP Factor Is Modulated by Blocking the Recruitment of Cullin 1 to the SCF Complex. Mol Cell Biol. 36, 3075-3085 (2016)(PMID:27697867)
Immunoprecipitation - Ogi T et al. Identification of the first ATRIP-deficient patient and novel mutations in ATR define a clinical spectrum for ATR-ATRIP Seckel Syndrome. PLoS Genet. 8, e1002945 (2012)(PMID:23144622)
- Mukai R, Ohshima T. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. 33, 2317-28 (2014)(PMID:23708656)
- Zhang HH et al. Nemo-like kinase is critical for p53 stabilization and function in response to DNA damage. Cell Death Differ. 21, 1656-63 (2014)(PMID:24926618)
Immunocytochemistry - Kitao T et al. Legionella Manipulates Non-canonical SNARE Pairing Using a Bacterial Deubiquitinase. Cell Rep. 32, 108107 (2020)
(PMID:32905772)
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