Antibody detailed product information

Code No. M132-3
Anti-HA-tag mAb
Size 100 µL (2 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 26, 2024 in JST）
Clonality Monoclonal
Clone 5D8
Isotype
(Immunized Animal) 		Mouse IgG1 κ
Applications WB　1 µg/mL  
IP　1 µg/sample  
Co-IP*　reported.  （PMID: 20506502 / 21586271 / 27697867）
Immunogen
(Antigen)		 Carrier protein conjugated peptide (CP-YPYDVPDYA)
Storage buffer 2 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Background Epitope tagging has widely been accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow; YPYDVPDYA (HA-tag), EQKLISEEDL (Myc-tag) and YTDIEMNRLGK (VSV-G-tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.
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561 Anti-HA-tag pAb
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Product category Tools:Epitope tags
Data


Citations

Western Blotting

  1. Ohshima T et al. HTLV‐1 basic leucine‐zipper factor, HBZ, interacts with MafB and suppresses transcription through a Maf recognition element. J Cell Biochem. 111, 187-94 (2010)(PMID:20506502)
  2. Kim TS et al. The ZFHX3 (ATBF1) transcription factor induces PDGFRB, which activates ATM in the cytoplasm to protect cerebellar neurons from oxidative stress. Dis Model Mech. 3, 752-62 (2010)(PMID:20876357)
  3. Mukai R, Ohshima T. Dual effects of HTLV-1 bZIP factor in suppression of interferon regulatory factor 1. Biochem Biophys Res Commun. 409, 328-32 (2011)(PMID:21586271)
  4. Mukai R, Ohshima T. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. 33, 2317-28 (2014)(PMID:23708656)
  5. Zhang HH et al. Nemo-like kinase is critical for p53 stabilization and function in response to DNA damage. Cell Death Differ. 21, 1656-63 (2014)(PMID:24926618)
  6. Mukai R, Ohshima T. Enhanced Stabilization of MCL1 by the Human T-Cell Leukemia Virus Type 1 bZIP Factor Is Modulated by Blocking the Recruitment of Cullin 1 to the SCF Complex. Mol Cell Biol. 36, 3075-3085 (2016)(PMID:27697867)
  7. Kitao T et al. Legionella Manipulates Non-canonical SNARE Pairing Using a Bacterial Deubiquitinase. Cell Rep. 32, 108107 (2020) (PMID:32905772)

Co-IP

  1. Ohshima T et al. HTLV‐1 basic leucine‐zipper factor, HBZ, interacts with MafB and suppresses transcription through a Maf recognition element. J Cell Biochem. 111, 187-94 (2010)(PMID:20506502)
  2. Mukai R, Ohshima T. Dual effects of HTLV-1 bZIP factor in suppression of interferon regulatory factor 1. Biochem Biophys Res Commun. 409, 328-32 (2011)(PMID:21586271)
  3. Mukai R, Ohshima T. Enhanced Stabilization of MCL1 by the Human T-Cell Leukemia Virus Type 1 bZIP Factor Is Modulated by Blocking the Recruitment of Cullin 1 to the SCF Complex. Mol Cell Biol. 36, 3075-3085 (2016)(PMID:27697867)

Immunoprecipitation

  1. Ogi T et al. Identification of the first ATRIP-deficient patient and novel mutations in ATR define a clinical spectrum for ATR-ATRIP Seckel Syndrome. PLoS Genet. 8, e1002945 (2012)(PMID:23144622)
  2. Mukai R, Ohshima T. HTLV-1 HBZ positively regulates the mTOR signaling pathway via inhibition of GADD34 activity in the cytoplasm. Oncogene. 33, 2317-28 (2014)(PMID:23708656)
  3. Zhang HH et al. Nemo-like kinase is critical for p53 stabilization and function in response to DNA damage. Cell Death Differ. 21, 1656-63 (2014)(PMID:24926618)

Immunocytochemistry

  1. Kitao T et al. Legionella Manipulates Non-canonical SNARE Pairing Using a Bacterial Deubiquitinase. Cell Rep. 32, 108107 (2020) (PMID:32905772)
  • The availability is based on the information in Japan at 00:05, Apr 26, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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