Availability (in Japan)
1-9
(In Japan at 17:00,
Feb 7, 2025 in JST)
Size
100 µL
Data | |||||
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Clonality | Polyclonal | Clone | polyclonal | ||
Isotype (Immunized Animal) | Rabbit Ig (aff.) | ||||
Applications | |||||
Immunogen (Antigen) | Recombinant Mouse Oct3/4 N-terminal (1-134 aa) | ||||
Reactivity [Gene ID] | |||||
Storage buffer | PBS/50% glycerol, pH 7.2 | ||||
Storage temp. | -20°C | Conjugate | Unlabeled | Manufacturer | MBL |
Alternative names | POU5F1, OCT3, OCT4, OTF3, OTF4, OTF-3 | ||||
Background | The POU family transcription factor Oct-3/4, termed as Oct-3 or Oct-4, encoded by Pou5f1, is expressed in totipotent/pluripotent early embryonic cells. It is also expressed in embryonic stem (ES) cells and embryonal carcinoma (EC) cells, but its expression diminishes when these cells differentiate and lose pluripotency. Oct3/4 contains three functionally characterized domains, the transcriptional activation domain of N- and C-terminal region and the POU DNA-binding domain. The POU domain binds to an octamer sequence, ATTTGCAT. Several target genes of Oct3/4, such as Sox2, contains an octamer element capable of binding Oct3/4. These sites are important for transcriptional activity. Induced pluripotent stem (iPS) cells can be generated from mouse embryonic or adult fibroblasts by induction of four factors, Oct3/4, Sox2, c-Myc, and Klf4. Oct3/4 regulates a expression of Tcl1 (T cell lymphoma break point) and Nanog and contributes to cell proliferation and stabilization of cell pluripotency. Two transcription factors Oct3/4 and Sox2 works together to control a transcriptional regulatory network that regulates the expression of other essential genes. | ||||
Related products | D356-3 Anti-Jmjd1c (Mouse) mAb PM056 Anti-Sox2 pAb PM057 Anti-KLF4 pAb PM058 Anti-Nanog (Mouse) pAb |
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Citations |
Immunocytochemistry
Immunohistochemistry
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Product category |
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