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MS160/Carboxyl & MS300/Carboxyl



MS160/Carboxyl & MS300/Carboxyl

Magnosphere™ MS160/Carboxyl & MS300/Carboxyl are magnetic particles especially for high purity bioseparation. The surface of these particles are covered with hydrophilic polymer for reducing non-specific binding. The carboxyl group allows the binding of the probe molecules.

Antibodies, proteins and nucleic acids can be immobilized on the surface for immunoassay, immunoprecipitation, western blot or nucleic acid hybridization.


Features and specifications

● Uniform particle diameter
● Superparamagnetic
● High-speed magnetic response
● Low non-specific binding

 MS160/CarboxylMS300/Carboxyl
Particle size 1.5 µm 3 µm
Surface carboxyl density 25 nmol/mg beads approx. 10 nmol/mg beads approx.
Ligand coupling Covalent coupling Covalent coupling
Application Immunoassay Immunoassay
Immnoprecipitaion

Application data 1 (Chemiluminescence enzyme immunoassay)

Chemiluminescence enzyme immunoassay (CLEIA) for prostate specific antigen (PSA) with MS160/Carboxyl


Chemiluminescence enzyme immunoassay (CLEIA) for prostate specific antigen (PSA) with MS300/Carboxyl


Application data 2 (Immunoprecipitation)

Immunoprecipitation of the 20S proteasome complex from Jurkat cell lysate

20S proteasome is a protein which composed of 28 subunits: 14 a-subunits and 14 b-subunits that form a four stacked ring. The anti-20S proteasome alpha 6 monoclonal antibody (Biomol International, L.P., clone MCP20) was conjugated to Magnosphere™ MS300/Carboxyl. The Magnosphere™ particles with the antibody was then used to immunoprecipitate the 20S proteasome complex from a Jurkat cell lysate (30 mg protein).
High-purity proteasome complexes (α1 ~ α7, β1 ~ β7) were recovered in Magnosphere™ MS300/Carboxyl.


Products List

Code No. Product Surface Diameter
(µm)
Function Density (COOH)
(µmol/g)
Particle Contents
(%)
Volume
(mL)
Preservative
J-MS-S160C MS160/Carboxyl Hydrophilic 1.5 25 1 4 Proclin 950
J-MS-S300C MS300/Carboxyl Hydrophilic 3 10 1 4 Proclin 950

Magnosphere is a trademark of JSR corporation.


 

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