HLA-A*24:02 cells (HLA-A*24:02 expressing C1R cells) were pulsed with or without survivin-2B80-88 peptide (10 µg/mL, AYACNTSTL) in RPMI 1640 medium containing 0.5% human serum albumin for 4 hours. The cells (1.0 × 106 cells/test) were stained with survivin-2B TCR Tetramer and anti-HLA-A24 antibody, as described in the Protocol. Numbers in the upper right quadrants represent the percentages of TCR Tetramer+ cells relative to the total HLA-A24+ cells.