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HOME > Product search results > Code No. U-N001-10
WNT Surrogate-Fc Fusion Protein
Code No. U-N001-10
WNT Surrogate-Fc Fusion Protein
Availability (in Japan)
0
(Contact us)
（In Japan at 17:00,
Dec 4, 2024 in JST）
Size
1 mg
Data Sheet
Use, storage and stability
Predilute the product to a 5 μM stock solution using a suitable buffer. The effective working concentration range varies between 0.1 - 0.5 nM and should be determined experimentally.
Undiluted Wnt Surrogate-Fc Fusion Protein should be stored at -80°C (stable for at least 1 year). The buffer contains 25 mM Tris 500 mM NaCl pH 8.2 without preservative. After thawing it should be stored in appropriate small aliquots at -20°C or -80°C (stable for at least 1 year).
Reconstituted Wnt Surrogate-Fc Fusion-protein in organoid culture medium is stable for 2 – 3 weeks at 4°C. Avoid repeated warm cycles to 37°C.
Production platform:
This product has been produced in HEK293 cells using the rPExTM recombinant protein production platform.
datasheet url: https://products.ipatherapeutics.com/product/n001-1-0mg/
Predilute the product to a 5 μM stock solution using a suitable buffer. The effective working concentration range varies between 0.1 - 0.5 nM and should be determined experimentally.
Undiluted Wnt Surrogate-Fc Fusion Protein should be stored at -80°C (stable for at least 1 year). The buffer contains 25 mM Tris 500 mM NaCl pH 8.2 without preservative. After thawing it should be stored in appropriate small aliquots at -20°C or -80°C (stable for at least 1 year).
Reconstituted Wnt Surrogate-Fc Fusion-protein in organoid culture medium is stable for 2 – 3 weeks at 4°C. Avoid repeated warm cycles to 37°C.
Production platform:
This product has been produced in HEK293 cells using the rPExTM recombinant protein production platform.
datasheet url: https://products.ipatherapeutics.com/product/n001-1-0mg/
| Intended use | stem cell culture; organoid | ||||
|---|---|---|---|---|---|
| Storage temp. | -80°C | Manufacturer | ImmunoPrecise Antibodies (Europe) BV | ||
| Alternative names | Surrogate Wnt agonist, Wnt | ||||
| Background | Description The Wnt signaling pathway is essential for embryonic development, stem cell differentiation and regeneration of injured tissues. Wnts hetero-dimerize Frizzled (Fzd) family receptors and their co-receptors Lrp5/6, triggering downstream signaling pathways including the canonical β-catenin cascade, and resulting in expression of genes regulating cell development. Wnt has been widely used as an essential cell growth factor in organoids. Currently, recombinant Wnt3a is commonly used as Wnt source. Unfortunately, recombinant Wnt3a is water insoluble and cannot serve as a medium supplement. Recombinant Wnt Surrogate-Fc Fusion-protein has an excellent solubility, and a better wnt response at lower effective concentrations than other sources of Wnt3a. WNT Surrogate-Fc Fusion-protein supports long-term expansion of multiple different types of organoids, including intestinal, kidney, colon, hepatocyte, ovarian, and breast organoids. |
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| Notes | When culturing organoids, stem cells, or other tissues, if you are to use this product in combination with other factor or factors (hereunder factors), a third party may have a patent on the use or other application of the factors concerned.Regarding to this product, we do not offer any non-infringement warranty when used or otherwise applied in combination with other factors. Therefore, if you intend to use this product in combination with other factors, please check with your organization’s division responsible for intellectual property rights or your research agency before using this product. | ||||
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