Availability (in Japan)
10 or more
（In Japan at 17:00,
Feb 26, 2024 in JST）
|stem cell culture; organoid culture
|roof plate-specific spondin 1, Rspo1, R-spondin 1, Cristin 3
|R-spondin 1 (roof plate-specific spondin 1), also known as Cristin 3, is one of the R-spondin family members that regulates to the activation of canonical WNT/β-catenin signaling.
In the absence of R-spondin, Frizzled (Fz) is endocytosed by being ubiquitinated by the binding of the ubiquitin ligase RNF43/ZNRF3, Fz on the cell membrane decreases, and Wnt signals are not activated enough.
Conversely, in the presence of R-spondin, RNF43/ZNRF3 forms trimers with LGR and R-spondin and is endocytosed, making it unable to ubiquitinate Fz. As a result, Fz on the cell membrane increases, and Wnt signals are strongly activated.
Organoid culture is typically utilized niche factors that act on Wnt, EGF, BMP and Notch signals that control stem cell differentiation. Among these signals, R-spondin 1 contributes to the activation of Wnt signals.
|When culturing organoids, stem cells, or other tissues, if you are to use this product in combination with other factor or factors (hereunder factors), a third party may have a patent on the use or other application of the factors concerned.Regarding to this product, we do not offer any non-infringement warranty when used or otherwise applied in combination with other factors. Therefore, if you intend to use this product in combination with other factors, please check with your organization’s division responsible for intellectual property rights or your research agency before using this product.