Anti-Fas (CD95) (Human) mAb

Availability (in Japan)

0
(Contact us)

（In Japan at 00:05,
Oct 8, 2024 in JST）

Size

100 µL (1 mg/mL)

Citations

Data Sheet

SDS

Clonality	Monoclonal	Clone	ZB4
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB 5 µg/mL FCM 5-20 µg/mL (final concentration)
Immunogen (Antigen)	Recombinant Human Fas
Reactivity [Gene ID]	Human[355]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	Fas (TNF receptor superfamily, member 6), APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6
Background	It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas (also known as CD95/APO-1) is a cell surface protein belonging to the tumor necrosis factor receptor superfamily, which is expressed in a variety of normal and neoplastic cells. Binding of FasL to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies results in rapid induction of apoptosis in Fas expressing cells. Clone ZB4 is known as Fas blocking, Fas neutralizing or Fas antagonistic antibody, whereas anti-Fas antibody (clone CH-11) is known as the agonistic antibody.
Related products	SY-001 Anti-Fas (CD95) (Human) mAb MD-10-3 Anti-Fas (CD95) (Human) mAb MD-10-4 Anti-Fas (CD95) (Human) mAb-FITC MD-10-5 Anti-Fas (CD95) (Human) mAb-PE
Citations	Zanin-Zhorov A et al. Fibronectin-associated Fas ligand rapidly induces opposing and time-dependent effects on the activation and apoptosis of T cells. J Immunol. 171, 5882-9 (2003)(PMID:14634098) Western Blotting Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893) Neutralization Chiou SH et al. Up-regulation of Fas ligand expression by human cytomegalovirus immediate-early gene product 2: a novel mechanism in cytomegalovirus-induced apoptosis in human retina. J Immunol. 167, 4098-103 (2001)(PMID:11564832) Chiou SH et al. The immediate early 2 protein of human cytomegalovirus (HCMV) mediates the apoptotic control in HCMV retinitis through up-regulation of the cellular FLICE-inhibitory protein expression. J Immunol. 177, 6199-206 (2006)(PMID:17056549) Function Jing ZT et al. Hepatitis B Virus Surface Antigen Enhances the Sensitivity of Hepatocytes to Fas-Mediated Apoptosis via Suppression of AKT Phosphorylation. J Immunol. 201, 2303-2314 (2018)(PMID:30171166)
Product category	Research area Apoptosis Cell surface antigens

The availability is based on the information in Japan at 00:05, Oct 8, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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