Anti-Podocalyxin (PCLP1) (Human) mAb-FITC

Availability (in Japan)

10 or more

（In Japan at 00:05,
Dec 5, 2024 in JST）

Size

100 µL (500 µg/mL)

Citations

Data Sheet

SDS

Data	Flow Cytometry
Clonality	Monoclonal	Clone	53D11
Isotype (Immunized Animal)	Mouse IgG2a
Applications	FCM 30 μg/mL (final concentration)
Immunogen (Antigen)	CHO cell expressing full-length human Podocalyxin/PCLP1
Reactivity [Gene ID]	Human[5420]
Storage buffer	500 µg/mL in PBS/1% BSA/0.09% NaN₃
Storage temp.	冷蔵	Conjugate	FITC	Manufacturer	MBL
Alternative names	PODXL, podocalyxin like, PC, PCLP, Gp200
Background	Recent studies with avian embryos and murine embryonic stem cells have suggested that hematopoietic cells are derived from hemangioblasts, the common precursors of hematopoietic and endothelial cells. Hara et al. molecularly cloned podocalyxin-like protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the AGM (aorta-gonad-mesonephros) region of mouse embryos, where long-term repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1⁺ CD45^- cells in the AGM region incorporated acetylated low-density lipoprotein and produced both hematopoietic and endothelial cells when cocultured with OP9 stromal cells. Moreover, multiple lineages of hematopoietic cells were generated in vivo when PCLP1⁺ CD45^- cells were injected into neonatal liver of busulfan-treated mice. Today it is reported that the PCLP1 is identical with the Podocalyxin
Related products	M084-3 Anti-Podocalyxin (PCLP1) (Human) mAb M084-5 Anti-Podocalyxin (PCLP1) (Human) mAb-PE
Citations	Flow Cytometry Kuhara M et al. Magnetic separation of human podocalyxin-like protein 1 (hPCLP1)-positive cells from peripheral blood and umbilical cord blood using anti-hPCLP1 monoclonal antibody and protein A expressed on bacterial magnetic particles. Cell Struct Funct. 34, 23-30 (2009)(PMID:19293534)
Product category	Research area Stem cell research Cell surface antigens

The availability is based on the information in Japan at 00:05, Dec 5, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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