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HOME > Product category > Neuroscience > Features of Acetylcholinesterase Rapid Staining Kit


Acetylcholinesterase Rapid Staining Kit

◎Nerves and ganglion cells are stained with this kit
◎Easy and Quick procedure
◎Kit utilizes enzymatic reaction of AChE

▼This Kit is used in the following research paper!
Kobayashi, H. et al. ; A new rapid acetylcholinesterase staining kit for diagnosing Hirschsprung's disease. Pediatr Surg Int. 23, 505-508 (2007)


Acetylcholinesterase (AChE) is a tetrameric serine hydrolase that rapidly catalyzes the hydrolysis of acetylcholine to acetate and choline.

The breakdown of acetylcholine is critical for the termination of impulse transmissions at cholinergic synapses within the nervous system. Progressive loss of cholinergic neurons in Alzheimer's disease (AD) patients results in severe memory loss and impairment of cognitive function. AChE inhibitors are a strategic approach to symptomatic treatment for AD, since AChE inhibitors increase the levels of acetylcholine in the synapse, thereby enhancing cholinergic activity in the affected regions of the brain.

AChE also plays an important role in agriculture since modifications in AChE can confer resistance to pesticides. AChE is a key component in many snake venoms, and AChE staining is routinely used for the initial diagnosis of Hirschsprung's disease, a congenital disorder caused by the absence of ganglion cells in the distal colon.

Example of Immunohistochemistry

Myenteric ganglia in normal intestine (x 200)

Submucosal hypertrophic nerve trunks in Hirschsprung's disease (x 400)

The photos are provided by Dr. Kobayashi Hiroyuki, Department of Pediatric Surgery, Juntendo University School of Medicine.


Staining procedure is easy and quick !
*Please refer to the data sheet for detailed procedure.

Kit Components

Code No. Product Size Price (Tax excluded)
8450 Acetylcholinesterase Rapid Staining Kit 30 tests ¥40,000
  • Solution A: 6 mL 1 vial
  • Solution B: 6 mL 1 vial
  • 3, 3'-diaminobenzidine (DAB) solution: 600 mL 1 vial
  • DAB dilution buffer: 12 mL 1 vial
  • Disposal droppers: 30 droppers


  • Kobayashi, H. et al., Pediatr Surg Int. 23: 505-508, 2007
  • Kobayashi H.,et al., Pediatr Surg Int 18:349-353, 2002
  • Kobayashi H.,et al., Arch Pathol Lab Med 118:1127-1129, 1994

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