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Reagents for COVID-19 Research



Last updated: Sep 16, 2020

Screening for COVID-19 T-cell peptides and immune monitoring with MHC tetramers in a single assay

COVID-19 infected cells can be recognized by T-cells only after SARS-CoV-2 peptides are processed and presented in the context of self MHCs.

Identifying these peptides have essential utilities:
▶Immune monitoring: Using peptide-MHC tetramers to assess vaccine-induced immunity
▶Designing potent vaccines that elicit durable responses

T-cells are activated only by interacting with processed peptides (e.g. of SARS-CoV-2) that sit in the groove of MHC class I and II molecules. Identifying these peptides can be used (in forms of peptide-MHC tetramers) to assess vaccine induced immunity and assist in designing potent vaccines with durable responses.

The protein sequence of SARS-CoV-2 is known and the predicted epitopes are identified and available published/reviewed by recent articles including one by Dr. Alessandro Sette:
A Sequence Homology and Bioinformatic Approach Can Predict Candidate Targets for Immune Responses to SARS-CoV-2
⇒ The predicted epitopes is launched! Affinities with MHCs has been evaluated using QuickSwitch™ Tetramer kits. Please find the peptide list!
Candidate targets for immune responses to 2019-Novel Coronavirus (nCoV): sequence homology- and bioinformatic-based predictions

We can assist companies and academic investigators who are actively developing coronavirus vaccines with our MHC Tetramer reagents.

  • Answer to the inquiry, “Does your vaccine elicit T-cell responses in hosts?”
  • Validate peptide binding affinities to multiple MHC class I and II alleles.
  • Can be used in in vitro assays to identify immune-potentiating candidate agents for their ability to enhance vaccines potency.

Products/Services

By using these kits, you can identify/validate the binding of predicted peptide sequences of the COVID-19 viral proteins in most common alleles: H-2Kb, HLA-A2, A11, A24.
※QuickSwitch™ kits for MHC class II are coming soon.


We can manufacture custom MHC tetramers or biotinylated monomers of your interest.


SARS-CoV-2 MHC Tetramers★NEW

HLA-A*02:01 Tetramers loading four SARS-CoV-2 spike glycoprotein peptides are available. Tetramer positive populations were detected using human samples in publications(References)。

アリルペプチド配列PEAPCBV421Biotinylated
monomer
HLA-A*02:01RLNEVAKNL TB-0174-1 TB-0174-2 TB-0174-4 TB-0174-M
HLA-A*02:01KLPDDFTGCV TB-0175-1 TB-0175-2 TB-0175-4 TB-0175-M
HLA-A*02:01YLQPRTFLL TB-0176-1 TB-0176-2 TB-0176-4 TB-0176-M
HLA-A*02:01RLQSLQTYV TB-0177-1 TB-0177-2 TB-0177-4 TB-0177-M

SARS-CoV-2 predicted epitope peptides★NEW

52 peptides are now available. Affinities with MHCs has been evaluated using QuickSwitch™ Tetramer kits.
※Detailed information is available from links in the tables below.

◇ MHC class II
Code No.Peptide sequenceTarget regionAffinity with allele
DRB1*01:01DRB1*04:01DRB1*15:01
SPC001EAFEKMVSLLSVLLSSARS-CoV-2 ORF1ab polyprotein (3906-3920)+++
SPC002EEIAIILASFSASTSSARS-CoV-2 ORF1ab polyprotein (471-485)+++
SPC003ESPFVMMSAPPAQYESARS-CoV-2 ORF1ab polyprotein (1801-1815)+++
SPC004FYVYSRVKNLNSSRVSARS-CoV-2 envelope protein (56-70)+++
SPC005LEASFNYLKSPNFSKSARS-CoV-2 ORF1ab polyprotein (2211-2225)+++
SPC006LLLLDRLNQLESKMSSARS-CoV-2 nucleocapsid phosphoprotein (221-235) ++
SPC007LSYYKLGASQRVAGDSARS-CoV-2 membrane glycoprotein (176-190)+++
SPC008MDLFMRIFTIGTVTLSARS-CoV-2 ORF3a protein (1-15)+++
SPC009MWLSYFIASFRLFARSARS-CoV-2 membrane glycoprotein (91-105)+++
SPC010RAMPNMLRIMASLVLSARS-CoV-2 ORF1ab polyprotein (5016-5030)+++
SPC011SEFSSLPSYAAFATASARS-CoV-2 ORF1ab polyprotein (3946-3960)+++
SPC012TRFQTLLALHRSYLTSARS-CoV-2 chain A spike glycoprotein (236-250) +++
SPC013WPQIAQFAPSASAFFSARS-CoV-2 nucleocapsid phosphoprotein (301-315) +++
SPC014YFTSDYYQLYSTQLSSARS-CoV-2 ORF3a protein (206-220)++
SPC046ADYSVLYNSASFSTFSARS-CoV-2 chain A spike glycoprotein (363-377) +++
SPC047IWLGFIAGLIAIVMVSARS-CoV-2 surface glycoprotein (1216-1230) ++
SPC048LAFVVFLLVTLAILTSARS-CoV-2 envelope protein (21-35) +
SPC049MKIILFLALITLATCSARS-CoV-2 ORF7a protein (1-15) ++
SPC050NRNRFLYIIKLIFLWSARS-CoV-2 membrane glycoprotein (41-55) +++
SPC051RVVVLSFELLHAPATSARS-CoV-2 chain A spike glycoprotein (509-523) +++
SPC052TQDLFLPFFSNVTWFSARS-CoV-2 chain A spike glycoprotein (51-65) +++

■Figure showing peptide exchange rate determined by QuickSwitch™ Tetramer kits
※QuickSwitch™ kits for MHC class II are coming soon.


Click to enlarge


◇ MHC class I
Code No.Peptide sequenceTarget regionAffinity with allele
A*02:01A*03:01A*11:01A*24:02
SPC015FLAHIQWMVSARS-CoV-2 ORF1ab (3122-3130) +
SPC016FLLNKEMYLSARS-CoV-2 ORF1ab (3183-3191) +
SPC017LLLDDFVEIISARS-CoV-2 ORF1ab (6749-6758) +
SPC018LLYDANYFLSARS-CoV-2 ORF3a protein (139-147) ++
SPC019SMWALIISVSARS-CoV-2 ORF1ab (3732-3740) +
SPC020TLMNVLTLVSARS-CoV-2 ORF1ab (3710-3718) +
SPC021YLDAYNMMISARS-CoV-2 ORF1ab (6419-6427) +
SPC022YLNTLTLAVSARS-CoV-2 ORF1ab (6851-6859) +
SPC023YLYALVYFLSARS-CoV-2 ORF3a protein (107-115) +
SPC024ASMPTTIAKSARS-CoV-2 ORF1ab (2192-2200) ++
SPC025KSAGFPFNKSARS-CoV-2 ORF1ab (4892-4900) ++
SPC026KTFPPTEPKSARS-CoV-2 nucleocapsid phosphoprotein (362-370) ++
SPC027STFNVPMEKSARS-CoV-2 ORF1ab (2600-2608) ++
SPC028TTIKPVTYKSARS-CoV-2 ORF1ab (1875-1883) ++
SPC029DYVYNPFMISARS-CoV-2 ORF1ab (6159-6167) +
SPC030FYGGWHNMLSARS-CoV-2 ORF1ab (4986-4994) +
SPC031NYLKRRVVFSARS-CoV-2 ORF1ab (3159-3167) +
Code No.Peptide sequenceTarget regionAffinity with allele
H-2KbHLA-A*02:01
SPC032VNFNFNGLChain A, SARS-CoV-2 spike glycoprotein (539-546)+
SPC033MAYRFNGIChain A, SARS-CoV-2 spike glycoprotein (902-909)+
SPC034INITRFQTLChain A, SARS-CoV-2 spike glycoprotein (233-241)+
SPC035VVLSFELLChain A, SARS-CoV-2 spike glycoprotein (511-518)+
SPC036SIVRFPNIChain A, SARS-CoV-2 spike glycoprotein (325-332)+
SPC037GNYNYLYRLChain A, SARS-CoV-2 spike glycoprotein (447-455)+
SPC038VVFLHVTYVChain A, SARS-CoV-2 spike glycoprotein (1060-1068)++
SPC039SIIAYTMSLChain A, SARS-CoV-2 spike glycoprotein (691-699)++
SPC040KLPDDFTGCVChain A, SARS-CoV-2 spike glycoprotein (424-433)+
SPC041VTQLYLGGMORF1ab polyprotein SARS-CoV-2 (5385-5393)+
SPC042ISDEFSSNVORF1ab polyprotein SARS-CoV-2 (5583-5591)+
SPC043ITGLYPTLORF1ab polyprotein SARS-CoV-2 (5573-5580) Peptide+
SPC044AAYYVGYLChain A, SARS-CoV-2 spike glycoprotein (263-270) Peptide+
SPC045YNYLYRLFChain A, SARS-CoV-2 spike glycoprotein (449-456) Peptide+

References
  • ・Poluektov Y et al., Assessment of SARS-CoV-2 Specific CD4(+) and CD8 (+) T Cell Responses Using MHC Class I and II Tetramers. bioRxiv. doi: 10.1101/2020.07.08.194209 (2020)
  • ・Herrmann A et al., A Targeted Vaccine against COVID-19: S1-Fc Vaccine Targeting the Antigen-Presenting Cell Compartment Elicits Protection against SARS-CoV-2 Infection. bioRxiv. doi: 10.1101/2020.06.29.178616 (2020)
  • ・Zhang Y et al., The ORF8 Protein of SARS-CoV-2 Mediates Immune Evasion through Potently Downregulating MHC-I. bioRxiv. doi: 10.1101/2020.05.24.111823 (2020)
  • ・Alina S. Shomuradova AS, et al., SARS-CoV-2 epitopes are recognized by a public and diverse repertoire of human T-cell receptors. bioRxiv. doi: 10.1101/2020.05.20.20107813 version 2 (2020)
  • ・Chour W et al., Shared Antigen-specific CD8+ T cell Responses Against the SARS-COV-2 Spike Protein in HLA A*02:01 COVID-19 Participants. bioRxiv. doi: 10.1101/2020.05.04.20085779 (2020)
  • ・Wang B et al., Identification of an HLA-A*0201–restricted CD8+ T-cell epitope SSp-1 of SARS-CoV spike protein. Blood. 104, 200–206 (2004) PMID: 15016646



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