QuickSwitch™ Custom Tetramer Kits
Example of specific CTL detection using peptide-exchanged Tetramer
HLA-A*02:01 CMV pp65 (NLVPMVATV) tetramers
Cells were stained with tetramer prepared using QuickSwitch™ Tetramer Kit or with MBL’s equivalent tetramer product. The peptide exchange efficiency was 92.5% (data not shown).
HLA-A*02:01 CMV pp65 (NLVPMVATV) and HLA-A*02:01 Influenza M1 (GILGFVFTL) tetramers
Human PBMCs were stained with tetramer prepared using QuickSwitch™ Tetramer Kit or with MBL's equivalent tetramer product (the number in the upper right corner of each panel indicates the percentage of tetramer-positive cells that are also CD8-positive).
The peptide exchange effi ciency of influenza M1 (GILGFVFTL) was 89% (data not shown).
H-2Kb OVA (SIINFEKL) tetramers
Spleens were harvested from OT-I TCR transgenic mice, and splenocytes (1.2×105 cells/test) were stained with 0.5 or 0.1 µg of tetramer reagents.
Green: H-2Kb TRP-2 Tetramer (negative control)
Red: H-2Kb OVA SIINFEKL Tetramer (prepared using QuickSwitch™ Tetramer kit)
Blue: H-2Kb OVA SIINFEKL Tetramer (MBL's equivalent tetramer product)
