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HOME > Product category > Immunology > Anti-HLA class I(HLA-A,B,C)antibody

Anti-HLA class I (HLA-A,B,C) antibody



Purpose

Immune checkpoint inhibitors and cancer vaccines are currently attracting a lot of attention as novel cancer treatment strategies. In order to achieve sufficient anti-tumor effects using these treatments, target tumor cells need to express HLA class I antigens. It is important to evaluate the expression of HLA class I antigens in tumor cells in order to establish treatment methods. This product is an antibody that recognizes human HLA-A,B,C. Because it can be used for immunostaining, it is useful for evaluating the expression of HLA class I in tumor cells and tissues.

  • Commonly used clone which has many citations
  • The product can be used for various applications

Application data

Western Blotting

Analysis of reactivity using recombinant MHC class I heavy chain extracellular domain

Analysis of HLA class I expression in oral cavity cancer cell line, OSC20

  Lane 1: Oral cavity cancer cell line OSC20
  Lane 2: OSC20-A*24:02 (HLA-A*24:02 transfectant)
  Lane 3: K562
  Lane 4: Recombinant HLA-A*24:02
  Data was kindly provided by Dr. Toshihiko Torigoe
  (The Department of Pathology, Sapporo Medical University School of Medicine).


Flow cytometry



  • The best clone for immunohistochemistry
  • The product is ready to use

Application data

Immunohistochemistry (fluorescent staining manual method)


HLA class I expression is observed in stromal cells, normal epithelial cells, infiltrating lymphocytes and macrophages. However, it is hardly observed on cancer cells (cytokeratin positive). HLA-DR is expressed on monocytes and macrophages.

Tissue:Head and neck cancer
Orange:HLA class I (Code No. D370-3H)
Red:HLA-DR (clone LN-3)
Blue:Cytokeratin (clone AE1/AE3)
Data was kindly provided by Dr. Susumu Suzuki (Aichi Medical University of Medicine).


Immunohistochemistry (Automated stainer BOND RX [Leica Biosystems])
  • Kidney cancer
  • Colorectal cancer
  • Breast cancer


Immunohistochemistry (Automated stainer VENTANA BenchMark XT [Roche Diagnostics])
  • Kidney cancer
  • Colorectal cancer
  • Breast cancer





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