Kit, protein, vector, etc detailed product information

Code No. U-R005-05
mRSPO1-his
Use, storage and stability
Mouse RSPO1-his protein has been successfully used in various types of organoids like colon, ileum and duodenum organoids. The working concentration of the product in organoid cultures should be determined experimentally. As a starting concentration 10-20 nM can be used. Depending on the type of organoid 0.2 nM WNT surrogate-Fc fusion protein (catalog U-N001), or similar, can be added.

The product should be stored at -80°C. After thawing it should be stored in appropriate small aliquots at -20°C or -80°C (stable for at least 6 months) or at 4°C for 3 weeks.

Production platform:
This product has been produced in HEK293 cells using the rPExTM recombinant protein production platform.

datasheet url: https://products.ipatherapeutics.com/product/r005-0-5-mg/
Size 0.5 mg
Availability (in Japan) 0
(Contact us)
(In Japan at 17:00, Dec 4, 2024 in JST)
Intended use stem cell culture; organoid
Storage temp. -80°C
Manufacturer ImmunoPrecise Antibodies (Europe) BV
Alternative names mRSPO1-his Protein, Mouse RPSO1-his, roof plate-specific spondin 1, Rspo1, R-spondin 1, Cristin 3
Background Description
RSPO1 is a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt/β-catenin signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Although R-spondins are unable to initiate Wnt signaling, they can potently enhance responses to low-dose Wnt proteins.
Notes When culturing organoids, stem cells, or other tissues, if you are to use this product in combination with other factor or factors (hereunder factors), a third party may have a patent on the use or other application of the factors concerned.Regarding to this product, we do not offer any non-infringement warranty when used or otherwise applied in combination with other factors. Therefore, if you intend to use this product in combination with other factors, please check with your organization’s division responsible for intellectual property rights or your research agency before using this product.
Product category Research area:Stem cell research Cytokine & Growth Factors Organoid
Tools:Cell culture reagents
  • The availability is based on the information in Japan at 17:00, Dec 4, 2024 in JST.
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  • For storage temparature: RT: room temparature
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