Kit, protein, vector, etc detailed product information

Code No.	U-R002-05
mRSPO1-Fc Fusion Protein
Use, storage and stability Mouse RSPO1-Fc fusion protein has been successfully used in various types of organoids like colon, ileum and duodenum organoids. The working concentration of the product in organoidcultures should be determined experimentally. As a starting concentration 10 – 20 nM can be used. Depending on the type of organoid 0.2 nM WNT surrogate-Fc fusion protein (catalog U-N001), or similar, can be added. The product should be stored at -80°C. After thawing it should be stored in appropriate small aliquots at -20°C or -80°C (stable for at least 6 months) or at 4°C for 3 weeks. Production platform: This product has been produced in HEK293 cells using the rPEx^TM recombinant protein production platform. datasheet url: https://products.ipatherapeutics.com/product/r002-0-5-mg/
Size	0.5 mg
Availability (in Japan)	0 (Contact us) （In Japan at 17:00, Dec 4, 2024 in JST）
Intended use	stem cell culture; organoid
Storage temp.	-80°C
Manufacturer	ImmunoPrecise Antibodies (Europe) BV
Alternative names	mRSPO1-Fc, mRSPO1-Fc Fusion, Mouse RPSO1-Fc fusion, roof plate-specific spondin 1, Rspo1, R-spondin 1, Cristin 3
Background	Description RSPO1 is a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt/β-catenin signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Although R-spondins are unable to initiate Wnt signaling, they can potently enhance responses to low-dose Wnt proteins.
Notes	When culturing organoids, stem cells, or other tissues, if you are to use this product in combination with other factor or factors (hereunder factors), a third party may have a patent on the use or other application of the factors concerned.Regarding to this product, we do not offer any non-infringement warranty when used or otherwise applied in combination with other factors. Therefore, if you intend to use this product in combination with other factors, please check with your organization’s division responsible for intellectual property rights or your research agency before using this product.
Product category	Research area:Stem cell research Cytokine & Growth Factors Organoid Tools:Cell culture reagents

The availability is based on the information in Japan at 17:00, Dec 4, 2024 in JST.
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