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HOME > Product search results > Code No. RN1007 BRIC Kit

Code No. RN1007

BRIC Kit

Availability (in Japan)

10 or more

(In Japan at 17:00,
Jun 4, 2020 in JST)

Size

20 assays

Data

  • BrU-labeled RNA was isolated by BRIC Kit from total RNA extracted after BrU pulse-chase labeling.


  • BrU-labeled RNA (BrU-RNA) was isolated by BRIC Kit from total RNA extracted at each time point after BrU washout. The isolated BrU-RNA was analyzed to determine its own half-life by RT-qPCR.

Components RNA-IP buffer, Wash buffer, mi-Solution I, mi-Solution II, mi-Solution III, mi-Solution IV, Protein G-Magnetic beads, BrU solution (100 mM), Anti-BrdU mAb, Spike-in control
Intended use The BRIC Kit is designed to analyze stability of RNA by chasing chronological decreases of BrU-labeled RNA in mammalian cells under physiologically undisturbed condition.
Storage temp. -20°C Manufacturer MBL
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Citations
  1. Machitani M et al. Dicer functions as an antiviral system against human adenoviruses via cleavage of adenovirus-encoded noncoding RNA.Sci Rep. 6, 27598 (2016)(PMID:27273616)
  1. Ueno D et al. Comprehensive analysis of mRNA internal cleavage sites in Arabidopsis thaliana. J Biol Bioeng. (2018) In press.(PMID:29358038)
  1. Yamazaki T et al. Functional Domains of NEAT1 Architectural lncRNA Induce Paraspeckle Assembly through Phase Separation. Mol Cell. 70, 1038-1053.e7. (2018)(PMID:29932899)
  1. Kami D et al. The DEAD-box RNA-binding protein DDX6 regulates parental RNA decay for cellular reprogramming to pluripotency. PLoS One. 13, e0203708 (2018)(PMID:30273347)
  1. Komatsu T et al. UPA-seq: prediction of functional lncRNAs using differential sensitivity to UV crosslinking. RNA 24, 1785-1802 (2018)(PMID:30232101)
  1. Joshi P et al. Transcriptome stability profiling identifies novel and functional MIRNA targets in melanoma cells. RNA Biology, in press.
References
  1. Tani H et al. Genome-wide determination of RNA stability reveals hundreds of short-lived noncoding transcripts in mammals. Genome Res. 22, 947-56 (2012)(PMID: 22369889
  2. Tani H, Akimitsu N. Genome-wide technology for determining RNA stability in mammalian cells: historical perspective and recent advantages based on modified nucleotide labeling. RNA Biol. 9, 1233-8 (2012)(PMID: 23034600
  3. Imamachi N et al. BRIC-seq: a genome-wide approach for determining RNA stability in mammalian cells. Methods 67, 55-63 (2014)(PMID: 23872059
  4. Tani H et al. The RNA degradation pathway regulates the function of GAS5 a non-coding RNA in mammalian cells. PLoS One 8, e55684 (2013)(PMID: 23383264
  5. Tani H et al. Identification of hundreds of novel UPF1 target transcripts by direct determination of whole transcriptome stability. RNA Biol. 9, 1370-9 (2012)(PMID: 23064114
  6. Maekawa S et al. Analysis of RNA decay factor mediated RNA stability contributions on RNA abundance. BMC Genomics. 16, 154 (2015)(PMID: 25879614

Product category
Research area
RNA-RNP Network
Epigenetics
Brands
RiboCluster Profiler™
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  • The availability is based on the information in Japan at 17:00, Jun 4, 2020 in JST.
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  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.

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