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HOME > Product search results > Code No. CY-1251V2 CycLex® NAMPT Colorimetric Assay Kit Ver.2

Code No.　CY-1251V2

CycLex® NAMPT Colorimetric Assay Kit Ver.2

Availability (in Japan)

10 or more

（In Japan at 00:05,
Dec 5, 2024 in JST）

Size

100 Assays

Data

  • Experimental example

    Experimental example
    Measurement of endogenous NAMPT activity in immunoprecipitates using Anti-NAMPT (Human) mAb (MBL; code no. CY-M1035)

  • Effect of FK866 on NAMPT activity

    Effect of FK866 on NAMPT activity
    Effect of FK866 on NAMPT activity in an immunoprecipitate from Raji cell extracts with Anti-NAMPT (Human) mAb (MBL; code no. CY-M1035).

  • Time course of NAMPT activity

    Time course of NAMPT activity
    Time course of NAMPT activity in recombinant NAMPT using One-Step Assay Method.
Components NAMPT Assay Buffer (20X), WST-1, ADH (alcohol dehydrogenase), Diaphorase, EtOH Solution, ATP, NMNAT1, Nicotinamide, PRPP, NAMPT
Intended use This Kit detects nicotinamide phosphoribosyltransferase (NAMPT) activity in recombinant NAMPT or endogenous NAMPT immunoprecipitated from cell extract.
Storage temp. -80°C Manufacturer MBL
Sample Recombinant NAMPT, NAMPT immunoprecipitated from cell lysates
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CY-1151V2 CycLex® SIRT1/Sir2 Deacetylase Fluorometric Assay Kit Ver.2
CY-1152V2 CycLex® SIRT2 Deacetylase Fluorometric Assay Kit Ver.2
CY-1153V2 CycLex® SIRT3 Deacetylase Fluorometric Assay Kit Ver.2
CY-1156V2 CycLex® SIRT6 Deacetylase Fluorometric Assay Kit Ver.2
CY-1158V2 CycLex® HDAC8 Deacetylase Fluorometric Assay Kit Ver.2
CY-M1035 Anti-NAMPT mAb
Citations
  1. Ogino Y et al. Cross resistance to diverse anticancer nicotinamide phosphoribosyltransferase inhibitors induced by FK866 treatment. Oncotarget. 9, 16451-16461 (2018)(PMID:29662658)
  1. Korotchkina L et al. OT-82, a Novel Anticancer Drug Candidate That Targets the Strong Dependence of Hematological Malignancies on NAD Biosynthesis. Leukemia. in press(PMID:31896781)
References
  1. Vedantham S et al. Aldose reductase drives hyperacetylation of Egr-1 in hyperglycemia and consequent upregulation of proinflammatory and prothrombotic signals. Diabetes. 63, 761-74 (2014)（PMID: 24186862）
  2. Laiguillon MC et al. Expression and function of visfatin (Nampt), an adipokine-enzyme involved in inflammatory pathways of osteoarthritis. 16, R38 (2014)（PMID: 24479481）
  3. Adams DJ et al. NAMPT is the cellular target of STF-31-like small-molecule probes. ACS Chem Biol. 9, 2247-54 (2014)（PMID: 25058389）
Product category
Research area
Cancer
Metabolism
Drug discovery
Circadian rhythm
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