HeLa-S3 cells transiently expressing both Ash/FKBP12 and hAG/mTOR (FRB) were observed at 0 minute (left) and 12 minutes (right) after addition of 500 nM Rapamycic.
Components
pAsh/FKBP12 Plasmid, phAG/mTOR Plasmid
Intended use
Fluoppi : Ash-hAG [mTOR-FKBP12] contains two plasmids for detecting mTOR(FRB)-Rapamycin-FKBP12 ternary complex in living cells.
"Please note that the product will be shipped after we receive a completed and signed License Acknowledgement. Prior to shipping, our staff may contact you to verify the intended use of the product.
Koyano F et al. Ubiqutitin is phosphorylated by PINK1 to activate perkin. Nature. 510, 162-6 (2014)(PMID:24784582)
Yamano K et al. Site-specific Interaction Mapping of Phosphorylated Ubiquitin to Uncover Parkin Activation. J Biol Chem. 290, 25199-211 (2015)(PMID:26260794)
Asamitsu K et al. Quantification of the HIV transcriptional activator complex in live cells by image-based protein-protein interaction analysis. Genes Cells. 21, 706-16 (2016)(PMID:27193293)
Watanabe T et al. Genetic visualization of protein interactions harnessing liquid phase transitions. Sci Rep. 7, 46380 (2017)(PMID:28406179)
References
Choi J et al. Structure of the FKBP12-rapamycin complex interacting with the binding domain of human FRAP. Science 273, 239-42 (1996)(PMID: 8662507)