Antibody detailed product information

Code No. W253-3
Anti-Glypican 1 (Human) mAb
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 19, 2024 in JST）
Clonality Monoclonal
Clone 12C1E2F
Isotype
(Immunized Animal) 		Mouse IgG1κ
Applications FCM　1-10 µg/mL  
Immunogen
(Antigen)		 Human Glypican 1 expressed Ba/F3transfectants generated from SST-REX (signal sequence trap by retrovirus-mediated expression screening).
Reactivity [Gene ID]

Human[2817]
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names GPC1
Background Glypican 1 (GPC1) is a member of cell surface heparan sulfate proteoglycan family. GPC1 is a GPI-anchored type membrane protein and probably involves in the control of cell division and growth regulation. GPC1 also associates with fibrillar APP A-beta peptides in lipid rafts in Alzheimer disease brains. In pancreatic ductal adenocarcinoma, enhanced GPC1 expression is suggested to correlate with BMP and activin receptors. Down-regulation of GPC1 results in suppression of pancreatic cancer cell growth.
Related products W164-3 Anti-RHBDD3 (Human) mAb
M075-3 Mouse IgG1 (isotype control)
MTG-001 Clear Back (Human Fc receptor blocking reagent)
Product category Research area:Cancer
Tools:Flow cytometry reagents
Data

References
  1. Kojima T, Kitamura T. A signal sequence trap based on a constitutively active cytokine receptor. Nat Biotechnol. 17, 487-90 (1999)（PMID: 10331810）
  2. Cheng F et al. Suppression of amyloid beta A11 antibody immunoreactivity by vitamin C: possible role of heparan sulfate oligosaccharides derived from glypican-1 by ascorbate-induced, nitric oxide (NO)-catalyzed degradation. J Biol Chem. 286, 27559-72 (2011)（PMID: 21642435）
  3. Svensson G, Mani K. S-Nitrosylation of secreted recombinant human glypican-1. Glycoconj J. 26, 1247-57 (2009)（PMID: 19479373）
  4. Mani K et al. Constitutive and vitamin C-induced, NO-catalyzed release of heparan sulfate from recycling glypican-1 in late endosomes. Glycobiology 16, 1251-61 (2006)（PMID: 16971378）
  5. Watanabe N et al. Glypican-1 as an Abeta binding HSPG in the human brain: its localization in DIG domains and possible roles in the pathogenesis of Alzheimer's disease. FASEB J. 18, 1013-5 (2004)（PMID: 15084524）
  6. Vermeesch SA et al. Assignment of the human glypican gene (GPC1) to 2q35-q37 by fluorescence in situ hybridization. Genomics 25, 327-9 (1995)（PMID: 7774946）
  7. David G et al. Molecular cloning of a phosphatidylinositol-anchored membrane heparan sulfate proteoglycan from human lung fibroblasts. J Cell Biol. 111, 3165-76 (1990)（PMID: 2148568）
  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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