Antibody detailed product information

Code No.	W052-3
Anti-Osteopontin (SPP1) (Human) mAb
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 00:05, Jun 7, 2026 in JST）
Clonality	Monoclonal
Clone	4A7D
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	FCM　1-10 µg/mL
Immunogen (Antigen)	Human Osteopontin (SPP1) expressed Ba/F3 transfectants generated from SST-REX (signal sequence trap by retrovirus-mediated expression screening).
Reactivity [Gene ID]	Human[6696]
Storage buffer	"1 mg/mL in PBS/50% glycerol, pH 7.2"
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	"OPN, BNSP, BSPI, ETA-1"
Background	"Osteopontin, also known as SPP1, is a secreted phosphoglycoprotein that belongs to the SIBLING family. Osteopontin binds to hydroxyapatite and is involved in anchoring of osteoclasts to the mineral matrix of bones. It also acts as a cytokine that mediates type I immune responses through enhancing the production of interferon-gamma and interleukin-12 while reducing production of interleukin-10. Osteopontin has been identified as a biomarker for ovarian cancer, prostate cancer, and non-small cell lung cancer."
Related products	M076-3 Mouse IgG2a (isotype control) MTG-001 Clear Back (Human Fc receptor blocking reagent) D050-3 Anti-CD29 (Integrin β1) (Human) mAb W164-3 Anti-RHBDD3 (Human) mAb
Product category	Research area:Stem cell research Cancer Cytokine & Growth Factors Tools:Flow cytometry reagents
Data
References	Kojima T, Kitamura T. A signal sequence trap based on a constitutively active cytokine receptor. Nat Biotechnol 17, 487-90 (1999)（PMID: 10331810）

The availability is based on the information in Japan at 00:05, Jun 7, 2026 in JST.
The special price is shown in red color.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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