Antibody detailed product information

Code No. SY-001
Anti-Fas (CD95) (Human) mAb
Size 100 µL (500 µg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 16, 2024 in JST）
Clonality Monoclonal
Clone CH-11
Isotype
(Immunized Animal) 		Mouse IgM
Applications WB　20 µg/mL  
FCM　20 µg/mL (final conc.)  
Function　Induction of apoptosis  
Immunogen
(Antigen)		 human diploid fibroblast FS-7 cell line
Reactivity [Gene ID]

Human[355], Mouse(-)
Storage buffer 500 µg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names FAS1, APT1, TNFRSF6, APO-1, FASTM, ALPS1A
Background It is now widely accepted that apoptosis plays an important role in the selection of immature thymocytes and Ag-primed peripheral T cells. Fas antigen is a cell-surface protein that mediates apoptosis. It is expressed in various tissues including the thymus and has structural homology with a number of cell-surface receptors, including tumor necrosis factor receptor and nerve growth factor receptor.
Related products MD-11-3 Anti-Fas (CD95) (Human) mAb
MD-10-3 Anti-Fas (CD95) (Human) mAb
MD-10-A48 Anti-Fas (CD95) (Human) mAb-Alexa Fluor™ 488
MD-10-4 Anti-Fas (CD95) (Human) mAb-FITC
MD-10-5 Anti-Fas (CD95) (Human) mAb-PE
M079-3 Mouse IgM (isotype control)
8442 MEBSTAIN Apoptosis TUNEL Kit III
M059-3 Anti-Caspase-10 (Human) mAb
D161-3 Anti-MFG-E8 (Mouse) mAb
Product category Research area:Apoptosis Cell surface antigens
Citations

Western Blotting

  1. Masuda A et al. Efficient Recruitment of c-FLIPL to the Death-Inducing Signaling Complex Leads to Fas Resistance in Natural Killer-Cell Lymphoma. Cancer Sci. 111, 807-816 (2020) (PMID:31908105)

Neutralization

  1. Takaki S, Eto K. Cytoplasmic localization of programmed cell death 4 contributes to its anti-apoptotic function. Mol Cell Biochem. 448, 155-164 (2018)(PMID:29442268)

Function

  1. Zanin-Zhorov A et al. Fibronectin-associated Fas ligand rapidly induces opposing and time-dependent effects on the activation and apoptosis of T cells. J Immunol. 171, 5882-9 (2003)(PMID:14634098)
  2. López-Huertas MR et al. The presence of HIV-1 Tat protein second exon delays fas protein-mediated apoptosis in CD4+ T lymphocytes: a potential mechanism for persistent viral production. J Biol Chem. 288, 7626-44 (2013)(PMID:23364796)
  3. Murthy A et al. A Crohn's disease variant in Atg16l1 enhances its degradation by caspase 3. Nature 506, 456-62 (2014)(PMID:24553140)
  4. Ma L et al. A novel small-molecule tumor necrosis factor α inhibitor attenuates inflammation in a hepatitis mouse model. J Biol Chem. 289, 12457-66 (2014)(PMID:24634219)
  5. Liu W et al. Hepatitis B virus core protein inhibits Fas-mediated apoptosis of hepatoma cells via regulation of mFas/FasL and sFas expression. FASEB J. 29, 1113-23 (2015)(PMID:25466893)
  6. Yanagi T et al. PCTAIRE1-knockdown sensitizes cancer cells to TNF family cytokines. PLoS One 10, e0119404 (2015)(PMID:25790448)
  7. Cui X et al. Cytoplasmic myosin-exposed apoptotic cells appear with caspase-3 activation and enhance CLL cell viability. Leukemia 30, 74-85 (2016)(PMID:26220042)
  8. Tan L et al. CD95 Signaling Inhibits B Cell Receptor-Mediated Gammaherpesvirus Replication in Apoptosis-Resistant B Lymphoma Cells. J Virol. 90, 9782–9796 (2016)(PMID:27558422)
  9. Hashiramoto A et al. A variant of death-receptor 3 associated with rheumatoid arthritis interferes with apoptosis-induction of T cell. J Biol Chem. 293, 1933-1943 (2018)(PMID:29180447)
  10. Kozako T et al. Novel small molecule SIRT2 inhibitors induce cell death in leukemic cell lines. BMC Cancer. 18, 791 (2018)(PMID:30081901)
  11. Jing ZT et al. Hepatitis B Virus Surface Antigen Enhances the Sensitivity of Hepatocytes to Fas-Mediated Apoptosis via Suppression of AKT Phosphorylation. J Immunol. 201, 2303-2314 (2018)(PMID:30171166)
  12. Sekiguchi Y et al. The anti-cancer drug gefitinib accelerates Fas-mediated apoptosis by enhancing caspase-8 activation in cancer cells. J Toxicol Sci. 44, 435-440 (2019)(PMID:31168030)
  13. Liu W et al. PI3K/AKT inhibitors aggravate death receptor-mediated hepatocyte apoptosis and liver injury. Toxicol Appl Pharmacol. 381, 114729 (2019)(PMID:31445927)
  14. Dagenais-Lussier X et al. USP18 is a significant driver of memory CD4 T-cell reduced viability caused by type I IFN signaling during primary HIV-1 infection. PLoS Pathog. 15, e1008060 (2019)(PMID:31658294)
  15. Asakawa M et al. Upregulation of Cancer-Associated Gene Expression in Activated Fibroblasts in a Mouse Model of Non-Alcoholic Steatohepatitis. Sci Rep. 9, 19601 (2019)(PMID:31862949)
  16. Masuda A et al. Efficient Recruitment of c-FLIPL to the Death-Inducing Signaling Complex Leads to Fas Resistance in Natural Killer-Cell Lymphoma. Cancer Sci. 111, 807-816 (2020)(PMID:31908105)
  17. Aoki K et al. Coordinated changes in cell membrane and cytoplasm during maturation of apoptotic bleb. Mol Biol Cell. 31, 833-844 (2020)(PMID:32049595)
  18. Thiagarajan D et al. Immunoglobulin G1 Antibodies Against Phosphorylcholine Are Associated With Protection in Systemic Lupus Erythematosus and Atherosclerosis: Potential Underlying Mechanisms. ACR Open Rheumatol. 2, 344-356 (2020)(PMID:32392632)
  19. Graeter S et al. Enhanced Pro-apoptotic Effects of Fe(II)-Modified IVIG on Human Neutrophils. Front Immunol. 11, 973 (2020)(PMID:32508840)
  20. Liu D et al. The protective role of miR-223 in sepsis-induced mortality. Sci Rep. 10, 17691 (2020) (PMID:33077816)
  • The availability is based on the information in Japan at 17:00, Apr 16, 2024 in JST.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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