Antibody detailed product information

Code No. RN019M
Anti-2,2,7-trimethylguanosine (m3G/TMG) mAb
Size 200 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Oct 9, 2024 in JST）
Clonality Monoclonal
Clone 235-1
Isotype
(Immunized Animal) 		Mouse IgG2aκ
Applications IP　10 µg/sample  
IC　0.5-1 µg/mL  
Immunogen
(Antigen)		 Carrier protein-conjugated trimethylguanosine (m3G/TMG)
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names N2,N2,7-Trimethylguanosine, m2,2,7G
Background Small nuclear RNA (snRNA) is known to be involved in pre-mRNA splicing as an RNA component of snRNP. 2,2,7-trimethylated guanosine (m3G/TMG) is a modified nucleoside at the 5’-cap site of U snRNAs except for U6 snRNA. Shortly after transcription, 7-methylguanosine (m7G), called cap structure, is added to 5’-end of newly synthesized U snRNA and m7G-capped U snRNA is exported to the cytoplasm. The m7G-cap is converted to m3G-cap, which is a hypermethylated structure of 5’-end, by cytoplasmic catalytic protein TSG1 and the m3G-capped U snRNA forms snRNP with Sm proteins in the cytoplasm, followed by entry into the nucleus. The m3G-capped snRNP plays a role in regulation of pre-mRNA splicing as a part of spliceosome in nucleus. It is also reported that U3 and U8 snoRNA, localized to Cajal body during RNP biogenesis, possess m3G-cap structure.
Related products RN1005 RIP-Assay Kit for microRNA
D347-3 Anti-Pseudouridine mAb
D345-3 Anti-1-methyladenosine (m1A) mAb
D346-3 Anti-5-methylcytidine (m5C) mAb
PM077 Anti-5-hydroxymethylcytosine (5hmC) pAb
MI-11-3 Anti-Bromodeoxyuridine mAb
MI-11-5 Anti-Bromodeoxyuridine mAb-PE
RN095PW Anti-SNRNP200 pAb
M218-3 Anti-5-hydroxymethylcytosine (5hmC) mAb
M227-3 Anti-Digoxigenin (DIG) mAb
M228-3 Anti-FITC mAb
RN077PW Anti-SMN1 pAb
RN124PW Anti-RNMT (Human) pAb
RN016M Anti-7-methylguanosine (m7G)-Cap mAb
RN017M Anti-7-methylguanosine (m7G) mAb
PM098 Anti-Inosine pAb
Product category Research area:RNA-RNP Network Epigenetics
Brands:RiboCluster Profiler™
Data


References
  1. Nottrott S et al. Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5' stem-loop of U4 snRNA. EMBO J. 18, 6119-33 (1999)（PMID: 10545122）
  2. Nagai K et al. Structure and assembly of the spliceosomal snRNPs. Novartis Medal Lecture. Biochem Soc Trans. 29, 15-26 (2001)（PMID: 11356120）
  3. Fox AH et al. Paraspeckles: a novel nuclear domain. Curr Biol. 12, 13-25 (2002)（PMID: 11790299）
  4. Yedavalli VS, Jeang KT. Trimethylguanosine capping selectively promotes expression of Rev-dependent HIV-1 RNAs. PNAS 107, 14787-92 (2010)（PMID: 20679221）
  5. Tsuiji H et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol. Med. 5, 221-34 (2013)（PMID: 23255347）
  • The availability is based on the information in Japan at 00:05, Oct 9, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
MBLCopyright (C)2015 Medical ＆ Biological Laboratories Co., Ltd. All Rights Reserve