Antibody detailed product information

Code No. RN016M
Anti-7-methylguanosine (m7G)-Cap mAb
This antibody (Clone 150-15) reacts with 5′-terminal 7-methylguanosine (m7G) cap structure of RNA and partially cross-reacts with m7G within RNA.
Size 200 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 20, 2024 in JST）
Clonality Monoclonal
Clone 150-15
Isotype
(Immunized Animal) 		Mouse IgG2aκ
Applications IP　10 µg/sample  
IC　Can be used.  
ELISA　Can be used.  
DB　1 µg/mL  
Immunogen
(Antigen)		 Carrier protein-conjugated 7-methylguanosine (m7G)-Cap analogue
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names 5’-cap, 7mGppp, m7G(5′)ppp(5′)X
Background The 7-methylguanosine (m7G) cap, also known as m7G(5′)ppp(5′)X, is a specific structure located on the 5′-terminus of the eukaryotic mRNA and in certain viral RNAs. This cap structure is also found at the 5′-terminus of mRNA-like lncRNA and pri-miRNA. The m7G cap is primarily involved in the translation of mRNAs. It is also responsible for the stability of the mRNA, and promotes transcription, splicing, polyadenylation, and nuclear export of the mRNA.
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Product category Research area:RNA-RNP Network Epigenetics
Brands:RiboCluster Profiler™
Data



References
  1. Rottman F et al. Sequences containing methylated nucleotides at the 5' termini of messenger RNAs: possible implications for processing. Cell 3, 197-9 (1974)（PMID: 4373171 ）
  2. Cowling VH. Regulation of mRNA cap methylation. Biochem J. 425, 295-302 (2009)（PMID: 20025612 ）
  3. Ramanathan A et al. mRNA capping: biological functions and applications. Nucleic Acids Res. (2016) In press.（PMID: 27317694 ）
  • The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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