Antibody detailed product information

Code No. RN004P
Anti-ELAVL1 (HuR) pAb
Size 200 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 19, 2024 in JST）
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal) 		Rabbit Ig (aff.)
Applications WB　1:1,000  
IP　5 µg/250 µL of cell extract from 2.5 x 106 cells  
IC　Can be used.  
RIP　15 µg/500 µL of cell extract from 1.5 x 107 cells  
Immunogen
(Antigen)		 KLH-conjugated synthetic peptide MSNGYEDHMAEDCRGDIGR (1-19 aa)
Reactivity [Gene ID]

Human[1994](PMID: 23176121), Mouse[15568], Rat[363854], Hamster[100754131]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R), HUR, Hua, MelG, ELAV1/HUR, W91709, 2410055N02Rik
Background ELAVL1/HuR, like other ELAVL proteins, has a short N-terminal region followed by 2 RNA-binding motifs, a basic linker domain, and a third RNA-binding motif. The basic hinge region bears a novel nuclear shuttling sequence which is implicated in the nucleocytoplasmic HuR shuttling. Unlike its neuron-specific ELAV relatives (HuB, HuC and HuD), HuR itself is ubiquitously expressed. Structure-function analyses of HuR have shown that it binds to AU-rich mRNA sequences in the nucleus and may be involved in their nuclear export.
Related products RN1001 RIP-Assay Kit
RN1005 RIP-Assay Kit for microRNA
RN005P Anti-ELAVL2 (HuB) (Human) pAb
RN006P Anti-ELAVL3 (HuC) pAb
RN007M Anti-ELAVL1 (HuR) mAb
Product category Research area:RNA-RNP Network Cancer
Brands:RiboCluster Profiler™
Data



Citations

Western Blotting

  1. Onomoto K et al. Critical role of an antiviral stress granule containing RIG-I and PKR in viral detection and innate immunity. PLoS One. 7, e43031 (2012)(PMID:22912779)

RNP Immunoprecipitation

  1. Williams TK et al. pp32 (ANP32A) expression inhibits pancreatic cancer cell growth and induces gemcitabine resistance by disrupting HuR binding to mRNAs. PLoS One. 5, e15455 (2010)(PMID:21152064)
  2. Pineda DM et al. HuR's post-transcriptional regulation of Death Receptor 5 in pancreatic cancer cells. Cancer Biol Ther. 13, 946-55 (2012)(PMID:22785201)
  3. Banadakoppa M et al. Role of transcription factor Sp1 and RNA binding protein HuR in the downregulation of Dr(+) Escherichia coli receptor protein decay accelerating factor (DAF or CD55) by nitric oxide. FEBS J. 280, 840-54 (2013)(PMID:23176121)
  4. Jinbo M et al. Targeting the mRNA-binding protein HuR impairs malignant characteristics of pancreatic ductal adenocarcinoma cells. Oncotarget 6, 27312-31 (2015)(PMID:26314962)
  5. Suhl JA et al. A 3' untranslated region variant in FMR1 eliminates neuronal activity-dependent translation of FMRP by disrupting binding of the RNA-binding protein HuR. PNAS 112, E6553-61 (2015)(PMID:26554012)
  6. Huang YH et al. Insights from HuR biology point to potential improvement for second-line ovarian cancer therapy. Oncotarget 7, 21812-24 (2016)(PMID:26943573)
  7. Romeo C et al. HuR contributes to TRAIL resistance by restricting Death Receptor 4 expression in pancreatic cancer cells. Mol Cancer Res. 14, 599-611 (2016)(PMID:27053682)
  8. Popovitchenko T et al. The RNA binding protein HuR determines the differential translation of autism-associated FoxP subfamily members in the developing neocortex. Sci. Rep. 6, 28998 (2016)(PMID:27383233)
  9. Zhou H et al. GPRC5A is a potential oncogene in pancreatic ductal adenocarcinoma cells that is upregulated by gemcitabine with help from HuR. Cell Death Dis. 7, e2294 (2016)(PMID:27415424)
  • The availability is based on the information in Japan at 17:00, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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