Antibody detailed product information

Code No.	PM097
Anti-NFIL3 (E4BP4) pAb
Size	100 µL
Availability (in Japan)	10 or more （In Japan at 00:05, Apr 19, 2024 in JST）
Clonality	Polyclonal
Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB　1 µg/mL ChIP*　（PMID: 31428688）
Reactivity [Gene ID]	Human[4783], Mouse[18030], Rat(-)
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	IL3BP1, NFIL3A, NF-IL3A
Background	NFIL3, or E4BP4, is a transcription factor that controls sleep and circadian rhythms throughout the cranial nerve, endocrine, and metabolic systems.The circadian clock is a 24-hour endogenous oscillator, which cycles via transcriptional/translational feedback loops of clock genes and their products in individual cells. CLOCK and BMAL1 are positive factors in the loop, and their complex binds to E-box elements of PERs and CRYs. PER and CRY bind the CLOCK-BMAL1 complex, suppressing E-box-dependent transactivation and forming a molecular clock via this negative feedback mechanism.D-box and REV-ERB/ROR-binding elements also form a regulatory network of clock-controlled genes (CCGs). D-box-dependent transactivation is activated by DBP and repressed by NFIL3/E4BP4.NFIL3/E4BP4 expression also plays an important role in the immune system. NFIL3 polymorphisms are associated with inflammatory bowel disease (IBD).
Related products	PM096 Anti-PER2 (Human) pAb D335-3 Anti-BMAL1 mAb D334-3 Anti-CLOCK (Mouse) mAb D333-3 Anti-CLOCK (Mouse) mAb PM079 Anti-DBP (Mouse) pAb PM075 Anti-GNAT2 (Zebrafish) pAb PM081 Anti-Cry1 (Mouse) pAb PM082 Anti-Cry2 (Mouse) pAb PM083 Anti-Per2 (Mouse) pAb PM087 Anti-Chrono (Mouse) pAb PM091 Anti-Per1 (Mouse) pAb PM092 Anti-NR1D1 (Rev-erbα) pAb PM093 Anti-NR1D2 (Rev-erbβ) pAb D349-3 Anti-CLOCK mAb M219-3 Anti-RORγt mAb M225-3 Anti-NFIL3 (E4BP4) chimeric mAb CY-1173 CycLex^® CaM-kinase II Assay Kit
Product category	Research area:Immunology Circadian rhythm
Data
Citations	Western Blotting Imamura K et al. ASK family kinases mediate cellular stress and redox signaling to circadian clock. PNAS (2018) In press.(PMID:29555767) Yoshitane H et al. Functional D-box sequences reset the circadian clock and drive mRNA rhythms. Commun Biol. 2, 300 (2019)(PMID:31428688) ChIP Yoshitane H et al. Functional D-box sequences reset the circadian clock and drive mRNA rhythms. Commun Biol. 2, 300 (2019)(PMID:31428688)
References	Yu X et al. Th17 cell differentiation is regulated by the circadian clock. Science 342, 727-30 (2013)（PMID: 24202171） Firth MA et al. Nfil3-independent lineage maintenance and antiviral response of natural killer cells. J Exp Med. 210, 2981-90 (2013) （PMID: 24277151） Kashiwada M et al. IL-4-induced transcription factor NFIL3/E4BP4 controls IgE class switching. PNAS 107, 821-6 (2010)（PMID: 20080759） Mitsui S et al. Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism. Genes Dev. 15, 995-1006 (2001)（PMID: 11316793） Ikushima S et al. Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes. PNAS 94, 2609-14 (1997)（PMID: 9122243） Zhang W et al. Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter. Mol Cell Biol. 15, 6055–63 (1995)（PMID: 7565758） Cowell IG et al. Transcriptional repression by a novel member of the bZIP family of transcription factors. Mol Cell Biol. 12, 3070-7 (1992)（PMID: 1620116）

The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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