Antibody detailed product information

Code No. PM056
Anti-Sox2 pAb
Size 100 µL
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 20, 2024 in JST）
Clonality Polyclonal
Clone Polyclonal
Isotype
(Immunized Animal) 		Rabbit Ig (aff.)
Applications WB　1:1,000  
IP　2 μL/300 μL of cell extract from 3 x 106 cells  
IC　1:200  
IH　1:500 (Heat treatment is necessary.）  
Immunogen
(Antigen)		 Partial recombinant mouse Sox2 protein
Reactivity [Gene ID]

Human[6657], Mouse[20674], Chicken*[396105](PMID: 23027349)
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names SRY (sex determining region Y)-box 2; ANOP3; MCOPS3
Background Sox2, a member of the SRY-related HMG-box (SOX) family of transcription factors, is expressed in embryonic inner cell mass, epiblast, extraembryonic mesoderm. It is one of the four factors (Oct3/4, Sox2, Klf4, c-Myc) that have been used to form iPS cells (induced pluripotent stem cells) from fibroblasts. Sox2 and Oct3/4 works together to control a transcriptional regulatory network that regulates the expression of other essential genes, and plays important roles in early embryonic development.
Related products PM048 Anti-Oct3/4 pAb
Product category Research area:Stem cell research
Data



Citations

Western Blotting

  1. Suzuki Y et al. SSEA-3 as a novel amplifying cancer cell surface marker in colorectal cancers. Int J Oncol. 42, 161-7 (2013)(PMID:23175153)
  2. Maekawa M et al. Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Nature 474, 225-9 (2011)(PMID:21654807)

Immunocytochemistry

  1. Gao Y et al. Isolation and biological characterization of chicken amnionnepithelial cells. Eur J Histochem. 56, e33 (20112)(PMID:23027349)

Immunohistochemistry

  1. Morishita S et al. Different Exercises Can Modulate the differentiation/maturation of Neural stem/progenitor Cells After Photochemically Induced Focal Cerebral Infarction. Brain Behav. 10, e01535 (2020)(PMID:31989796)
  2. Chiu YF et al. Critical role of SOX2-IGF2 signaling in aggressiveness of bladder cancer. Sci Rep. 10, 8261 (2020)(PMID:32427884)
References
  1. Niwa H. How is pluripotency determined and maintained? Development 134, 635-646 (2007) (PMID: 17215298)
  2. Yu J et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 318, 1917-20 (2007) (PMID: 18029452)
  3. Masui S et al. Pluripotency governed by Sox2 via regulation of Oct3/4 expression in mouse embryonic stem cells. Nat Cell Biol. 9, 625-35 (2007) (PMID: 17515923)
  4. Avilion AA et al. Multipotent cell lineages in early mouse development depend on SOX2 function. Genes Dev. 17, 126-40 (2003) (PMID: 12514105)
  • The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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