Antibody detailed product information

Code No.	PD027
Anti-Rubicon (Human) pAb
Size	100 µL
Availability (in Japan)	10 or more （In Japan at 17:33, Apr 26, 2024 in JST）
Clonality	Polyclonal
Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB　1:1,000 IP　5 µL/300 µL of cell extract from 3 x 10⁶ cells IH　Can be used.
Immunogen (Antigen)	Recombinant human Rubicon, C-terminal (722-972 aa)
Reactivity [Gene ID]	Human[9711], Mouse(-)
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	KIAA0226
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Rubicon was identified as Beclin1 interacting protein. Three distinct Beclin1 complexes exist in cells, one of the complexes including Rubicon (Beclin1, hVps34, hVps15, UVRAG, Rubicon) down regulates the process of autophagosome maturation and endocytosis.
Related products	M170-3 Anti-Rubicon (Human) mAb PD017 Anti-Beclin 1 pAb M160-3 Anti-UVRAG mAb PD026 Anti-Atg14 pAb M184-3 Anti-Atg14 (Human) mAb PM072 Anti-VMP1 pAb MD-06-3 Anti-PI3-kinase (p85α) mAb
Product category	Research area:Autophagy
Data
Citations	Western Blotting Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med.19, 1478-88 (2013)(PMID:24141421) Bejarano E et al. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 16, 401-14 (2014)(PMID:24705551)

The availability is based on the information in Japan at 17:33, Apr 26, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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