Antibody detailed product information

Code No.	PD026
Anti-Atg14 pAb
Size	100 µL
Availability (in Japan)	10 or more （In Japan at 00:05, Apr 26, 2024 in JST）
Clonality	Polyclonal
Clone	Polyclonal
Isotype (Immunized Animal)	Rabbit Ig (aff.)
Applications	WB　1:500 IP　5 µL/300 µL of cell extract from 3 x 10⁶ cells IC*　reported. （PMID: 24705551）
Immunogen (Antigen)	Recombinant Human Atg14 (167-404 a.a.)
Reactivity [Gene ID]	Human[22863], Mouse[100504663], Rat[305831], Hamster(-)
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	ATG14, autophagy related 14, ATG14L, BARKOR, KIAA0831/ Atg14L, 4832427M01, D14Ertd114e, D14Ertd436e
Background	Autophagy is a process of intracellular bulk degradation in which cytoplasmic components including organelles are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. Mammalian homologues of Atg14/Atg14L/BARKOR localizes on the isolation membrane and autophagosome, and it necessary for autophagosome formation. Atg14 binds to the PI3K complex (Beclin-1, Vps34, Vps15) and promotes the autophagosome formation.
Related products	M184-3 Anti-Atg14 (Human) mAb PD017 Anti-Beclin 1 pAb MD-06-3 Anti-PI3-kinase (p85α) mAb M170-3 Anti-Rubicon (Human) mAb PD027 Anti-Rubicon (Human) pAb M160-3 Anti-UVRAG mAb PM072 Anti-VMP1 pAb
Product category	Research area:Autophagy
Data
Citations	Western Blotting Hamasaki M et al. Autophagosomes form at ER-mitochondria contact sites. Nature 495, 389-93 (2013)(PMID:23455425) Maejima Y et al. Mst1 inhibits autophagy by promoting the interaction between Beclin1 and Bcl-2. Nat Med. 19, 1478-88 (2013)(PMID:24141421) Kim C, Bergelson JM. Echovirus 7 entry into polarized caco-2 intestinal epithelial cells involves core components of the autophagy machinery. J Virol. 88, 434-43 (2014)(PMID:24155402) Bejarano E et al. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 16, 401-14 (2014)(PMID:24705551) Immunoprecipitation Kim J et al. Differential regulation of distinct Vps34 complexes by AMPK in nutrient stress and autophagy. Cell 152, 290-303 (2013)(PMID:23332761) Zhong Y et al. Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels. J Biol Chem. 289, 26021-37 (2014)(PMID:25086043) Nemazanyy I et al. Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling. Nat Commun. 6, 8283 (2015)(PMID:26387534) Immunocytochemistry Bejarano E et al. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 16, 401-14 (2014)(PMID:24705551) Shizukuishi S et al. Streptococcus pneumoniae hijacks host autophagy by deploying CbpC as a decoy for Atg14 depletion. EMBO Rep. 21, e49232 (2020)(PMID:32239622)

The availability is based on the information in Japan at 00:05, Apr 26, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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