Antibody detailed product information

Code No.	MH-12-3
Anti-PCNA (Proliferating Cell Nuclear Antigen) mAb
Size	100 µL (1 mg/mL)
Availability (in Japan)	1-9 （In Japan at 17:02, Jul 17, 2026 in JST）
Clonality	Monoclonal
Clone	5A10
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB　5 µg/mL IP　5 µg/200 µL of cell extract from 2.5 x 10⁶ cells FCM　10-50 µg/mL (final concentration) IC　10 µg/mL IH　2 µg/mL (Heat treatment is necessary for paraffin embedded sections.)
Immunogen (Antigen)	Recombinant Rat PCNA
Reactivity [Gene ID]	Human[5111], Mouse[18538], Rat[25737]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Related products	D289-3 Anti-Np95 (Uhrf1) (Mouse) mAb M019-3 Anti-Nucleolin mAb M202-3 Anti-Np95 (Uhrf1) mAb
Product category	Research area:Cancer
Data
Citations	Western Blotting Kawasoe Y et al. MutSα maintains the mismatch repair capability by inhibiting PCNA unloading. Elife 5, e15155 (2016)(PMID:27402201) Zhu B et al. Baf53a is involved in survival of mouse ES cells, which can be compensated by Baf53b. Sci Rep. 7, 14059 (2017)(PMID:29070872) Wang Y et al. Deletion of INMAP postpones mitotic exit and induces apoptosis by disabling the formation of mitotic spindle. Biochem Biophys Res Commun. 518, 19-25 (2019)(PMID:31405563) Immunohistochemistry Chariyakornkul A et al. Low-polar extract from seed of Cleistocalyx nervosum var. paniala modulates initiation and promotion stages of chemically-induced carcinogenesis in rats. Biomed Pharmacother. 133, 110963 (2021) (PMID:33190034)

The availability is based on the information in Japan at 17:02, Jul 17, 2026 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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