Antibody detailed product information

Code No. M153-3
Anti-Atg5 mAb
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 19, 2024 in JST）
Clonality Monoclonal
Clone 4D3
Isotype
(Immunized Animal) 		Mouse IgG1 κ
Applications WB　2-5 µg/mL  
Immunogen
(Antigen)		 Recombinant Human full-length Atg5 (275 a.a.)
Reactivity [Gene ID]

Human[9474], Mouse[11793], Rat(-), Hamster[100759159]
Storage buffer PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names autophagy related 5, ASP, APG5, APG5L, hAPG5, APG5-LIKE
Related products 8485 Autophagy Ab Sampler Set
PM050 Anti-Atg5 pAb
M133-3 Anti-Atg3 mAb
PM039 Anti-Atg7 (Human) pAb
PM036 Anti-LC3 pAb
M152-3 Anti-LC3 mAb
M151-3 Anti-Atg10 (Human) mAb
M154-3 Anti-Atg12 (Human) mAb
PM040 Anti-Atg16L pAb
M150-3 Anti-Atg16L mAb
PD017 Anti-Beclin 1 pAb
PM072 Anti-VMP1 pAb
Notes This antibody detects Atg5-Atg12 complex (55 kDa).
Product category Research area:Autophagy
Data

Citations

Western Blotting

  1. Takahashi Y et al. Bif-1 regulates Atg9 trafficking by mediating the fission of Golgi membranes during autophagy. Autophagy. 7, 61-73 (2011)(PMID:21068542)
  2. Takaesu G et al. TGFβ-activated kinase 1 (TAK1)-binding proteins (TAB) 2 and 3 negatively regulate autophagy. J Biochem. 151, 157-66 (2012)(PMID:21976705)
  3. Young MM et al. Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J Biol Chem. 287, 12455-68 (2012)(PMID:22362782)
  4. Chen H et al. CD133/prominin-1-mediated autophagy and glucose uptake beneficial for hepatoma cell survival. PLoS One 8, e56878 (2013)(PMID:23437259)
  5. Guo X et al. Patulin induces pro-survival functions via autophagy inhibition and p62 accumulation. Cell Death Dis. 4, e822 (2013)(PMID:24091665)
  6. Katagiri N et al. The nucleolar protein nucleophosmin is essential for autophagy induced by inhibiting Pol I transcription. Sci Rep. 5, 8903 (2015)(PMID:25754892)
  7. Liu Y et al. Bif-1 deficiency impairs lipid homeostasis and causes obesity accompanied by insulin resistance. Sci Rep. 6, 20453 (2016)(PMID:26857140)
  8. Cheng J et al. Autophagy regulates MAVS signaling activation in a phosphorylation-dependent manner in microglia. Cell Death Differ. 24, 276-287 (2017)(PMID:28141795)
  9. Ye M et al. Curcumin Improves Palmitate-Induced Insulin Resistance in Human Umbilical Vein Endothelial Cells by Maintaining Proteostasis in Endoplasmic Reticulum. Front Pharmacol. 8, 148 (2017)(PMID:28377722)
  10. Mitroi DN et al.　SGPL1 (sphingosine phosphate lyase 1) modulates neuronal autophagy via phosphatidylethanolamine production.　Autophagy 13, 885-899 (2017)(PMID:28521611)
  11. Tang Z et al. Atg2A/B deficiency switches cytoprotective autophagy to non-canonical caspase-8 activation and apoptosis. Cell Death Differ. 24, 2127-2138 (2017)(PMID:28800131)
  12. Taniguchi K et al. Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence. Am J Cancer Res. 8, 650-661 (2018)(PMID:29736310)
  13. Scrivo A et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun. 10, 780 (2019)(PMID:30770803)

Immunocytochemistry

  1. Scrivo A et al. Gigaxonin E3 ligase governs ATG16L1 turnover to control autophagosome production. Nat Commun. 10, 780 (2019)(PMID:30770803)
  • The availability is based on the information in Japan at 00:05, Apr 19, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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