Antibody detailed product information

Code No.	M148-3M
Anti-monomeric Kusabira-Green N-terminal fragment mAb
Size	100 µL (1 mg/mL)
Availability (in Japan)	1-9 （In Japan at 00:05, Jun 9, 2026 in JST）
Clonality	Monoclonal
Clone	1E6
Isotype (Immunized Animal)	Mouse IgG2b
Applications	WB　1 µg/mL IC*　（PMID: 22911572）
Immunogen (Antigen)	mKG_N (1-168 a.a.)
Storage buffer	"1 mg/mL in PBS/50% glycerol, pH 7.2"
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	mKG_N
Background	"CoralHue^® Fluo-chase Kit can detect protein-protein interactions as fluorescent signals using the protein fragment complementation method. The gene of CoralHue^® monomeric Kusabira-Green (mKG), a reporter protein, is divided into two fragments (CoralHue^®mKG_N fragment and CoralHue^®mKG_C fragment) which are respectively fused to the target protein genes to investigate the interactions. When the expressed target proteins don’t interact, CoralHue^®mKG_N fragment and CoralHue^®mKG_C fragment cannot approach each other and can not emit fluorescence. However, when target proteins interact, divided CoralHue^®mKG fragments spatially approach each other and the local effective concentration increases. As a result, mKG fragments form a steric structure before dividing and the chromophore emits fluorescence. The fluorescent signals can be detected depending on the fused target protein-protein interactions."
Related products	M149-3M Anti-monomeric Kusabira-Green C-terminal fragment mAb M168-3M Anti-monomeric Kusabira-Orange 2 mAb
Notes	This antibody (clone 1E6) has the epitope on CoralHue^®mKG_N fragment and can detect the fusion protein with CoralHue^®mKG_N specifically.
Product category	Tools:Fluorescent proteins
Data
Citations	Immunocytochemistry Nitta S et al. Hepatitis C virus NS4B protein targets STING and abrogates RIG-I-mediated type I interferon-dependent innate immunity. Hepatology 57, 46-58 (2013)(PMID:22911572)

The availability is based on the information in Japan at 00:05, Jun 9, 2026 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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