Antibody detailed product information

Code No.	M074-3
Anti-Apaf-1 (Human) mAb
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 17:33, Aug 7, 2026 in JST）
Clonality	Monoclonal
Clone	5C1
Isotype (Immunized Animal)	Mouse IgG1
Applications	WB　5µg/mL
Immunogen (Antigen)	His-tagged human Apaf-1 fusion protein corresponding to N-terminal amino acids (1-421 a. a.)
Reactivity [Gene ID]	Human[317], Mouse(w)[11783], Rat(w)[78963]
Storage buffer	1.0 mg/mL in PBS/50% glycerol
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	apoptotic protease activating factor 1, CED4, APAF-1
Product category	Research area:Apoptosis
Data
References	Pan, G., et al., Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex. J. Biol. Chem. 237, 5841-5845 (1998)（PMID: 9488720） Yoshida, H., et al., Apaf1 is required for mitochondrial pathways of apoptosis and brain development. Cell 94, 739-750 (1998)（PMID: 9753321） Cecconi, F., et al., Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 94, 727-737 (1998)（PMID: 9753320） Adrain, C., et al., Regulation of apoptotic protease activating factor-1 oligomerization and apoptosis by the WD-40 repeat region. J. Biol. Chem. 274, 20855-20860 (1999)（PMID: 10409627） Perkins, C. L., et al., The role of Apaf-1, caspase-9, and bid proteins in etoposide- or paclitaxel-induced mitochondrial events during apoptosis. Cancer Res. 60, 1645-1653 (2000)（PMID: 10749135）

The availability is based on the information in Japan at 17:33, Aug 7, 2026 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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