Antibody detailed product information

Code No.	K0218-3
Anti-MICA/B (Human) mAb
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 00:05, Apr 20, 2024 in JST）
Clonality	Monoclonal
Clone	BAMO3
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	IP　2-5 µg/300 µL of cell extract FCM　10 µg/mL (final concentration) ELISA　1 µg/mL (for capture antibody)
Immunogen (Antigen)	MICA01, MICA04 and MICB*02 transfected P815 cells
Reactivity [Gene ID]	Human[4277]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Background	MICA and MICB (Major Histocompatibility Complex class I Chain-related gene A and gene B) bind to the activating immunoreceptor NKG2D. NKG2D is expressed on NK (Natural Killer) cells, NKT cells, γδT cells and CD8⁺αβT cells. Recognition of MICA and MICB by NKG2D is involved in tumor surveillance, immune responses to viral infections and autoimmune diseases. MICA and MICB are transmembrane glycoproteins that are distantly related to the MIC proteins, and they possess three extra-cellular Ig-like domains. And thus, MICA and MICB are closely related but are functionally indistinguishable. MICA and MICB molecules are highly glycosylated, and are detected as a smear band ranging from 65-75 kDa. It is reported that MICA and MICB are highly expressed in variant tumor cells, whereas normal cells express little. Tumor cells have been shown to shed and release MIC molecules from the cell surface. Therefore determination of soluble MIC (sMIC) levels provides valuable information for cancer staging, and sMIC in serum seems to be an indicator for systemic manifestation of malignancy rather than for local tumor extent.
Related products	K0217-3 Anti-MICA (Human) mAb K0219-3 Anti-MICA/B (Human) mAb K0220-3 Anti-MICB (Human) mAb M076-3 Mouse IgG2a (isotype control)
Product category	Research area:Immunology Cancer
Data

The availability is based on the information in Japan at 00:05, Apr 20, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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