Antibody detailed product information

Code No.	D291-A59
Anti-His-tag mAb-Alexa Fluor™ 594
Size	50 µg/50 µL
Availability (in Japan)	10 or more （In Japan at 17:01, Feb 16, 2026 in JST）
Clonality	Monoclonal
Clone	OGHis
Isotype (Immunized Animal)	Mouse IgG2aκ
Applications	IC　1 µg/mL IH*　reported. （PMID: 28890351）
Immunogen (Antigen)	6xHis tagged protein
Storage buffer	1 mg/mL in PBS/1% BSA/0.1% ProClin150
Storage temp.	4°C
Conjugate	Alexa Fluor® 594
Manufacturer	MBL
Background	The His-tag (6xHis-tag) is one of the most common tags used to facilitate the purification of recombinant proteins. Metal chelate affinity chromatography is widely used for purification of His-tagged proteins.　This specific antibody is useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of the recombinant proteins.
Related products	PM032 Anti-His-tag pAb D291-3 Anti-His-tag mAb D291-6 Anti-His-tag mAb-Biotin D291-7 Anti-His-tag mAb-HRP-DirecT D291-8 Anti-His-tag mAb-Agarose D291-10 Anti-His-tag mAb-Magnetic Agarose D291-A48 Anti-His-tag mAb-Alexa Fluor™ 488 D291-A64 Anti-His-tag mAb-Alexa Fluor™ 647 M089-3 Anti-His-tag mAb M136-3 Anti-His-tag mAb D291-11 Anti-His-tag mAb-Magnetic Beads
Product category	Tools:Epitope tags
Data
Citations	Immunocytochemistry Ungerer C et al. Galectin-9 is a suppressor of T and B cells and predicts the immune modulatory potential of mesenchymal stromal cell preparations. Stem Cells Dev. 23, 755-66 (2014)(PMID:24083426) Yang C et al. Role of Suppressor of Cytokine Signaling 3 in the Immune Modulation of Mesenchymal Stromal Cells. Inflammation 39, 257-68 (2016)(PMID:26318865) Immunohistochemistry Kikuchi T et al. In situ delivery and production system of trastuzumab scFv with Bifidobacterium. Biochem Biophys Res Commun. 493, 306-312 (2017)(PMID:28890351)

The availability is based on the information in Japan at 17:01, Feb 16, 2026 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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