Code No. |
D199-3 |
Anti-MFG-E8 (Mouse) mAb
|
Size |
100 µL (1 mg/mL) |
Availability (in Japan) |
1-9
(In Japan at 17:33, Apr 26, 2024 in JST)
|
Clonality |
Monoclonal |
Clone |
18A2-G10 |
Isotype
(Immunized Animal) |
Hamster IgG |
Applications |
WB 1 µg/mL IH 1 µg/mL (Can be used in cryosections.)
|
Immunogen
(Antigen) |
Recombinant Mouse MFG-E8 |
Reactivity [Gene ID] |
Mouse[17304] |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2 |
Storage temp. |
-20°C |
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
P47, MP47, Mfgm, SED1, lactadherin |
Background |
Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and binds to phosphatidylserine exposed on the cell surface of apoptotic cells, then binds strongly to cells expressing avb3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages. |
Related products |
D161-3 Anti-MFG-E8 (Mouse) mAb
|
Product category |
Research area:Apoptosis Exosomes |
Data |
|
Citations |
Western Blotting - Aziz MM et al. Prolactin induces MFG-E8 production in macrophages via transcription factor C/EBPbeta-dependent pathway. Apoptosis. 13, 609-20 (2008)(PMID:18392683)
- Hoffhines AJ et al. Tyrosylprotein sulfotransferase-2 expression is required for sulfation of RNase 9 and Mfge8 in vivo. J Biol Chem. 284, 3096-105 (2009)(PMID:19047058)
- Bobrie A et al. Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. J Extracell Vesicles. 1, 18397 (2012)(PMID:24009879)
- Aziz M et al. Milk fat globule-epidermal growth factor-factor 8 attenuates neutrophil infiltration in acute lung injury via modulation of CXCR2. J immunol. 189, 393-402 (2012)(PMID:22634615)
Neutralization - Fujiwara et al. The significance of tumor cells-derived MFG-E8 in tumor growth of angiosarcoma. J Dermatol Sci. 96, 18-25 (2019)(PMID:31447183)
Immunohistochemistry - Jinushi M et al. MFG-E8-mediated uptake of apoptotic cells by APCs links the pro- and antiinflammatory activities of GM-CSF. J Clin Invest. 117, 1902-13 (2007)(PMID:17557120)
- Kranich J et al. Follicular dendritic cells control engulfment of apoptotic bodies by secreting Mfge8. J Exp Med. 205, 1293-302 (2008)(PMID:18490487)
- Middendorp S et al. Mice deficient for CD137 ligand are predisposed to develop germinal center-derived B-cell lymphoma. Blood. 114, 2280-9 (2009)(PMID:19608748)
- Yoshino M et al. Analysis of capturing skin antigens in the steady state using milk fat globule EGF factor 8-deficient skin-hyperpigmented mice. Immunol Lett. 115, 131-37 (2008)(PMID:18083239)
- Abe T et al. Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8. J Immunol. 193, 1383-91 (2014)(PMID:24958900)
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