Antibody detailed product information

Code No. D190-3
Anti-Aggrus (Podoplanin) (Mouse) mAb
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 17:00, Apr 20, 2024 in JST）
Clonality Monoclonal
Clone 8F11
Isotype
(Immunized Animal) 		Rat IgG2a
Applications WB　1 µg/mL  
IP　1-2 µg/250 µL of cell extract from 2.5 x 106 cells  
FCM　5-10 µg/mL (final concentration)  
IC*　reported.  （PMID: 18924607）
IH　1.25 µg/mL (whole mount)  
Immunogen
(Antigen)		 NL-17 cell membrane fraction
Reactivity [Gene ID]

Mouse[14726]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names Pdpn, T1a, Gp38, OTS-8, T1alpha, RANDAM-2, T1-alpha, PA2.26 antigen, glycoprotein 38, transmembrane glycoprotein E11
Background In addition to hemostasis and host defense, platelets are involved in the induction of inflammation, tissue repair, and tumor metastasis. Aggrus (T1α/Podoplanin) is a novel 36-45 kDa membrane sialoglycoprotein that induces platelet aggregation. Aggrus is highly expressed in various tumor cells and the platelet aggregation ability of Aggrus depends on specific oligosaccharide structures that may be related to the metastasis process in cancer cells. Aggrus has been identified as a diagnostic tumor marker for seminomas and testicular cancers.
Related products D321-A48 Anti-Podoplanin (Mouse) mAb -Alexa Fluor™ 488
D321-3 Anti-Podoplanin (Mouse) mAb
Product category Research area:Cancer Cell surface antigens
Data




Citations

Western Blotting

  1. Chida J et al. Prion protein protects mice from lethal infection with influenza A viruses. PLoS Pathog. 14, e1007049 (2018)(PMID:29723291)
  2. Kadowaki H et al. Molecular mechanism of ER stress-induced pre-emptive quality control involving association of the translocon, Derlin-1, and HRD1. Sci Rep. 8, 7317 (2018)(PMID:29743537)
  3. Chida J et al. Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice. PLoS Pathog. 16, e1008823 (2020)(PMID:32845931)

Flow Cytometry

  1. Lapinski PE et al. RASA1 maintains the lymphatic vasculature in a quiescent functional state in mice. J Clin Invest. 122, 733-47 ((2012)(PMID:22232212)

Immunocytochemistry

  1. Fu J et al. Endothelial cell O-glycan deficiency causes blood/lymphatic misconnections and consequent fatty liver disease in mice. J Clin Invest. 118, 3725-37 (2008)(PMID:18924607)

Immunohistochemistry

  1. Kallert SM et al. Replicating viral vector platform exploits alarmin signals for potent CD8+ T cell-mediated tumour immunotherapy. Nat Commun. 8, 15327 (2017)(PMID:28548102)
  2. Chida J et al. Prion protein protects mice from lethal infection with influenza A viruses. PLoS Pathog. 14, e1007049 (2018)(PMID:29723291)
  • The availability is based on the information in Japan at 17:00, Apr 20, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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