Antibody detailed product information

Code No.	D187-3
Anti-Dlk (Pref-1) mAb
Size	100 µL (1 mg/mL)
Availability (in Japan)	10 or more （In Japan at 17:00, Apr 25, 2024 in JST）
Clonality	Monoclonal
Clone	24-11
Isotype (Immunized Animal)	Rat IgG1
Applications	FCM　5-10 µg/mL IC*　reported. （PMID: 23943621） IH　10 µg/mL (paraffin), 1 µg/mL (frozen)
Immunogen (Antigen)	Dlk-Fc fusion protein
Reactivity [Gene ID]	Mouse[13386]
Storage buffer	1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp.	-20°C
Conjugate	Unlabeled
Manufacturer	MBL
Alternative names	delta-like 1 homolog (Drosophila), FA1, ZOG, pG2, DlkI, Peg9, SCP1, DLK-1, Ly107, pref-1, AW742678
Background	Delta like protein (Dlk), also known as Preadipocyte factor-1 (Pref-1) or zona glomerulosa-specific factor (ZOG), is an EGF-like transmembrane protein expressed preadipocytes but not in mature adipocytes. It is highly expressed in fetal liver, the adrenal gland, and placenta, as well as some neuroendocrine tumors and small cell lung carcinomas, where it plays a role in differentiation and proliferation. Dlk positively and negatively regulates adipocyte differentiation via at least four major variants (45-60 kDa) of Dlk generated by alternatively splicing. Constitutive expression of Dlk inhibits adipogenesis, but insulin or insulin like growth factor-1 (IGF-1) can circumvent this inhibition. Regulated processing of Dlk releases a 50 kDa soluble form that was previously characterized as Fetal Antigen-1, a protein involved in pancreatic island cell differentiation.
Related products	D187-4 Anti-Dlk (Pref-1) mAb-FITC D187-5 Anti-Dlk (Pref-1) mAb-PE
Product category	Research area:Stem cell research Cancer
Data
Citations	Flow Cytometry Khurana S, Mukhopadhyay A. Hematopoietic progenitors from early murine fetal liver possess hepatic differentiation potential. Am J Pathol. 173, 1818-27 (2008)(PMID:18988804) Cheung PF et al. Granulin-epithelin precursor is an oncofetal protein defining hepatic cancer stem cells. PLoS One 6, e28246 (2011)(PMID:22194816) Kanayama K et al. Genome-Wide Mapping of Bivalent Histone Modifications in Hepatic Stem/Progenitor Cells. Stem Cells Int. 2019:9789240 (2019) (PMID:31065285) Immunocytochemistry García-Alonso V et al. Coordinate functional regulation between microsomal prostaglandin E synthase-1 (mPGES-1) and peroxisome proliferator-activated receptor γ (PPARγ) in the conversion of white-to-brown adipocytes. J Biol Chem. 288, 28230-42 (2013)(PMID:23943621) Immunohistochemistry Tan KS et al. Localized SCF and IGF-1 secretion enhances erythropoiesis in the spleen of murine embryos. Biol Open 4, 596-607 (2015)(PMID:25887124) Tan KS et al. Analysis of Hematopoietic Niche in the Mouse Embryo. Methods Mol Biol. 2002, 13-27 (2019)(PMID:30671734)

The availability is based on the information in Japan at 17:00, Apr 25, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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