Code No. | D138-3 | |
Anti-Sap155 mAb | ||
Size | 100 µL (1 mg/mL) | |
Availability (in Japan) | 1-9
(In Japan at 17:33, Apr 26, 2024 in JST) |
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Clonality | Monoclonal | |
Clone | 1A5 | |
Isotype (Immunized Animal) |
Mouse IgG2b | |
Applications | WB 1 µg/mL |
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Immunogen (Antigen) |
Recombinant Mouse Sap155 | |
Reactivity [Gene ID] | ||
Storage buffer | 1 mg/mL in PBS/50% glycerol, pH 7.2 | |
Storage temp. | -20°C | |
Conjugate | Unlabeled | |
Manufacturer | MBL | |
Alternative names | SF3B1, MDS, PRP10, Hsh155, PRPF10, SAP155, SF3b155 | |
Background | SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the major spliceosome. SF3 contains the Spliceosome-Associated Proteins, SAP 49, 130, 145, and 155. SAP155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) spliceosome. SAP155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two-hybrid system. SAP155 contains numerous Cdk consensus phosphorylation sites in its N terminus and is phosphorylated prior to catalytic step II of the splicing pathway. SAP155 serves as a substrate for cyclin E-cdk2 in vitro, suggesting that pre-mRNA splicing may be linked to the cell cycle machinery in mammalian cells. | |
Related products | D221-3 Anti-Sap155 mAb D139-3 Anti-Ring1B mAb RN085PW Anti-U2AF1 pAb |
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Product category | Research area:RNA-RNP Network Epigenetics Cancer | |
Data | ||
Citations | Western Blotting
Immunoprecipitation
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