Antibody detailed product information

Code No. D058-11
Anti-Multi Ubiquitin mAb-Magnetic Beads
Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono ubiquitinylated proteins but not free ubiquitin (PMID:18757370, 19237541) .
Size 20 tests (Slurry: 1 mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 25, 2024 in JST）
Clonality Monoclonal
Clone FK2
Isotype
(Immunized Animal) 		Mouse IgG1 κ
Applications IP　50 µL of beads slurry/sample  
Immunogen
(Antigen)		 a crude poly-ubiquitin-lysozyme
Reactivity [Gene ID]

Human
Storage buffer 5 mg magnetic beads in 1mL PBS/0.1% BSA/0.09% NaN3
Storage temp. 4°C
Conjugate Magnetic Beads
Manufacturer MBL
Background Ubiquitin is a polypeptide of 76 amino acid residues, and widely distributed protein in eukaryotic cells. This protein is also highly conserved among eukaryotic cells. Recently several reports showed that intracellular abnormal and short-lived proteins are degradated through an ubiquitin dependent proteolytic pathway. In the ubiquitin dependent pathway, a target protein is tagged with multi-ubiquitin molecules.
Related products D058-3 Anti-Multi Ubiquitin mAb
D058-8 Anti-Multi Ubiquitin mAb-Agarose
M075-11 Mouse IgG1 (isotype control)-Magnetic Beads
Product category Research area:Ubiquitin proteasome
Brands:Smart-IP
Data

References
  1. Fujimuro M et al. Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins. FEBS Lett. 349, 173-180 (1994)（PMID: 7519568）
  2. Takada K et al. Immunoassay for the quantification of intracellular multi-ubiquitin chains. Eur J Biochem. 233, 42-47 (1995)（PMID: 7588772）
  • The availability is based on the information in Japan at 00:05, Apr 25, 2024 in JST.
  • The special price is shown in red color.
  • Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
  • Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
MBLCopyright (C)2015 Medical ＆ Biological Laboratories Co., Ltd. All Rights Reserve