Antibody detailed product information

Code No. D055-3
Anti-M6a (Mouse) mAb
Size 100 µL (1 mg/mL)
Availability (in Japan) 10 or more
（In Japan at 00:05, Apr 26, 2024 in JST）
Clonality Monoclonal
Clone 321
Isotype
(Immunized Animal) 		Rat IgG2aκ
Applications FCM　5-10 µg/mL (final concentration)  
IH　1-10 µg/mL  
IF*　reported.  （PMID: 18241840 / 28851962）
Other(magnetic cell separation)　reported.  （PMID: 26702136）
Other(Transmission electron microscopy (TEM))　reported.  （PMID: 28851962）
Immunogen
(Antigen)		 Mouse M6a expressing cells
Reactivity [Gene ID]

Mouse[234267]
Storage buffer 1 mg/mL in PBS/50% glycerol, pH 7.2
Storage temp. -20°C
Conjugate Unlabeled
Manufacturer MBL
Alternative names Gpm6a
Background M6 is a membrane glycoprotein that is abundantly expressed on central neurons in the CHS. Two distinct murine M6 cDNA (designated M6a and M6b) whose deduced amino acid sequences are remarkably similar to that of the major CNS myelin protein PLP/DM20 are known. PLP expression is limited to the white matter. M6a appears in post-mitotic neurons of the brain and spinal cord as early as embryonic day 10, and later in the hippocampus, cerebral cortex, and the granule cells of the cerebellum. In contrast, M6b is expressed at early embryonic stage in the ventricular zone of the spinal cord, and later during development in both neurons and glia.
Product category Research area:Stem cell research Neuroscience Exosomes
Data

Citations

Flow Cytometry

  1. Lua I et al. Characterization of hepatic stellate cells, portal fibroblasts, and mesothelial cells in normal and fibrotic livers. J Hepatol. 64, 1137-1146 (2016)(PMID:26806818)

Immunocytochemistry

  1. Aparicio GI et al. Identification of Potential Interacting Proteins With the Extracellular Loops of the Neuronal Glycoprotein M6a by TMT/MS. Front Synaptic Neurosci. 12, 28 (2020)(PMID:32848694)

Immunohistochemistry

  1. Cooper B et al. Glycoprotein M6a is present in glutamatergic axons in adult rat forebrain and cerebellum. Brain Res. 1197, 1-12 (2008)(PMID:18241840)
  2. Zhao J et al. M6a is expressed in the murine neural retina and regulates neurite extension. Mol Vis. 14, 1623-30 (2008)(PMID:18776950)
  3. Dorrell C et al. Isolation of mouse pancreatic alpha, beta, duct and acinar populations with cell surface markers. Mol Cell Endocrinol. 339, 144-50 (2011)(PMID:21539888)
  4. Lua I et al. Myofibroblastic Conversion and Regeneration of Mesothelial Cells in Peritoneal and Liver Fibrosis. Am J Pathol. 185, 3258-73 (2015)(PMID:26598235)
  5. Li Y et al. Role of TGF-β signaling in differentiation of mesothelial cells to vitamin A-poor hepatic stellate cells in liver fibrosis. Am J Physiol Gastrointest Liver Physiol. 310, G262-72 (2016)(PMID:26702136)

Immunofluorescence

  1. Cooper B et al. Glycoprotein M6a is present in glutamatergic axons in adult rat forebrain and cerebellum. Brain Res. 1197, 1-12 (2008)(PMID:18241840)
  2. Monteleone MC et al. Neural glycoprotein M6a is released in extracellular vesicles and modulated by chronic stressors in blood. Sci Rep. 7, 9788 (2017)(PMID:28851962)

Other(magnetic cell separation)

  1. Li Y et al. Role of TGF-β signaling in differentiation of mesothelial cells to vitamin A-poor hepatic stellate cells in liver fibrosis. Am J Physiol Gastrointest Liver Physiol. 310, G262-72 (2016)(PMID:26702136)

Other(Transmission electron microscopy (TEM))

  1. Monteleone MC et al. Neural glycoprotein M6a is released in extracellular vesicles and modulated by chronic stressors in blood. Sci Rep. 7, 9788 (2017)(PMID:28851962)
  • The availability is based on the information in Japan at 00:05, Apr 26, 2024 in JST.
  • The special price is shown in red color.
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  • Abbreviations for applications：
    WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
    FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
    ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
    DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization
  • For applications and reactivity:
    *: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
    **: The use is reported from the licenser (Under evaluation or not tested by MBL).
  • For storage temparature: RT: room temparature
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