Antibody detailed product information
| Code No. | 554 | |
| Anti-p27Kip1 pAb | ||
| Size | 100 µL | |
| Availability (in Japan) | 1-9
（In Japan at 17:33, Apr 26, 2024 in JST） |
|
| Clonality | Polyclonal | |
| Clone | Polyclonal | |
| Isotype (Immunized Animal) |
Rabbit Ig (aff.) | |
| Applications | WB 1:100-1:1,000 IP 5 μL/100 μL of cell extract from 5x106 cells FCM 1:100 (final concentration) IC 1:100 |
|
| Immunogen (Antigen) |
Recombinant Mouse p27kip1 | |
| Reactivity [Gene ID] | ||
| Storage buffer | PBS/50% glycerol, pH 7.2 | |
| Storage temp. | -20°C | |
| Conjugate | Unlabeled | |
| Manufacturer | MBL | |
| Alternative names | CDKN1B | |
| Background | Cyclin dependent kinase inhibitory protein 1 (Kip1) is a 27 kDa inducible inhibitor of cyclin-dependent kinase complexes (CKI). It acts by suppressing S-phase entry and thereby blocking cell cycle progression. Kip1 interacts strongly with D-type cyclins in complex with Cdk4 and weaklier with cyclin E/Cdk2 complex to cause G1 arrest. Kip1 is a potent tumor suppressor for multiple epithelially derived neoplasias, and expression of Kip1 is frequently down regulated in human tumors. Kip1 levels are regulated by phosphorylation and degradation via the ubiquitin-proteosome pathway. | |
| Product category | Research area:Cancer | |
| Data |  |
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- The availability is based on the information in Japan at 17:33, Apr 26, 2024 in JST.
- The special price is shown in red color.
- Please note that products cannot be ordered from this website. To purchase the items listed in this website, please contact us or local distributers.
- Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
DB: Dot Blotting, NB: Northern Blotting, RNA FISH: RNA Fluorescence in situ hybridization - For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
- For storage temparature: RT: room temparature
- Please note that products in this website might be changed or discontinued without notification in advance for quality improvement.
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