Anti-Glypican 1 (Human) mAb

Availability (in Japan)

10 or more

（In Japan at 00:05,
Apr 18, 2024 in JST）

Size

100 µL (1 mg/mL)

Data Sheet

SDS

Data	Flow Cytometry
Clonality	Monoclonal	Clone	12C1E2F
Isotype (Immunized Animal)	Mouse IgG1κ
Applications	FCM 1-10 µg/mL
Immunogen (Antigen)	Human Glypican 1 expressed Ba/F3transfectants generated from SST-REX (signal sequence trap by retrovirus-mediated expression screening).
Reactivity [Gene ID]	Human[2817]
Storage buffer	PBS/50% glycerol, pH 7.2
Storage temp.	-20°C	Conjugate	Unlabeled	Manufacturer	MBL
Alternative names	GPC1
Background	Glypican 1 (GPC1) is a member of cell surface heparan sulfate proteoglycan family. GPC1 is a GPI-anchored type membrane protein and probably involves in the control of cell division and growth regulation. GPC1 also associates with fibrillar APP A-beta peptides in lipid rafts in Alzheimer disease brains. In pancreatic ductal adenocarcinoma, enhanced GPC1 expression is suggested to correlate with BMP and activin receptors. Down-regulation of GPC1 results in suppression of pancreatic cancer cell growth.
Related products	W164-3 Anti-RHBDD3 (Human) mAb M075-3 Mouse IgG1 (isotype control) MTG-001 Clear Back (Human Fc receptor blocking reagent)
References	Kojima T, Kitamura T. A signal sequence trap based on a constitutively active cytokine receptor. Nat Biotechnol. 17, 487-90 (1999)（PMID: 10331810） Cheng F et al. Suppression of amyloid beta A11 antibody immunoreactivity by vitamin C: possible role of heparan sulfate oligosaccharides derived from glypican-1 by ascorbate-induced, nitric oxide (NO)-catalyzed degradation. J Biol Chem. 286, 27559-72 (2011)（PMID: 21642435） Svensson G, Mani K. S-Nitrosylation of secreted recombinant human glypican-1. Glycoconj J. 26, 1247-57 (2009)（PMID: 19479373） Mani K et al. Constitutive and vitamin C-induced, NO-catalyzed release of heparan sulfate from recycling glypican-1 in late endosomes. Glycobiology 16, 1251-61 (2006)（PMID: 16971378） Watanabe N et al. Glypican-1 as an Abeta binding HSPG in the human brain: its localization in DIG domains and possible roles in the pathogenesis of Alzheimer's disease. FASEB J. 18, 1013-5 (2004)（PMID: 15084524） Vermeesch SA et al. Assignment of the human glypican gene (GPC1) to 2q35-q37 by fluorescence in situ hybridization. Genomics 25, 327-9 (1995)（PMID: 7774946） David G et al. Molecular cloning of a phosphatidylinositol-anchored membrane heparan sulfate proteoglycan from human lung fibroblasts. J Cell Biol. 111, 3165-76 (1990)（PMID: 2148568）
Product category	Research area Cancer Tools Flow cytometry reagents

The availability is based on the information in Japan at 00:05, Apr 18, 2024 in JST.
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Abbreviations for applications：
WB: Western Blotting, IH: Immunohistochemistry, IC: Immunocytochemistry, IP: Immunoprecipitation
FCM: Flow Cytometry, NT: Neutralization, IF: Immunofluorescence, RIP: RNP Immunoprecipitation
ChIP: Chromatin Immunoprecipitation, CoIP: Co-Immunoprecipitation
For applications and reactivity:
*: The use is reported in a research article (Not tested by MBL). Please check the data sheet for detailed information.
**: The use is reported from the licenser (Under evaluation or not tested by MBL).
For storage temparature: RT: room temparature
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