Data |
Western Blotting
Immunoprecipitation
RNP Immunoprecipitation
|
Clonality |
Polyclonal
|
Clone |
Polyclonal |
Isotype
(Immunized Animal)
|
Rabbit Ig (aff.)
|
Applications |
- WB
- 1 µg/mL
- IP
- 5 µg/500 µL of cell extract from 1 x 107 cells
- IC
- reported (PMID: 21935400 / 26990993)
- RIP
- 15 µg/500 µL of cell extract from 1.3 x 107 cells
- Other(eCLIP)
- reported. (PMID: 27018577)
|
Immunogen
(Antigen) |
KLH conjugated synthetic peptide, corresponding to internal region of human TNRC6A
|
Reactivity
[Gene ID] |
Human[27327], Mouse(-), Rat(-) |
Storage buffer |
1 mg/mL in PBS/50% glycerol, pH 7.2
|
Storage temp. |
-20°C
|
Conjugate |
Unlabeled |
Manufacturer |
MBL |
Alternative names |
GW1, TNRC6, CAGH26
|
Background |
The trinucleotide repeat containing 6A (TNRC6A), also known as GW182, was so named because of its molecular weight and the presence of multiple glycine (G)–tryptophan (W) amino acid pairs in the N-terminal region that is required for interaction with Argonaute proteins. GW182 is a critical component of cytoplasmic processing bodies (P-bodies), which have been shown to function in mRNA degradation, storage, and miRNA-mediated gene silencing. The interaction of GW182 with Argonautes is required for P-body localization and may be critical for miRNA-mediated translational repression, because it induces the formation of the complexes containing miRNA–target mRNA. Further, the phosphorylated form of human TNRC6A has been shown to play a role in miRNA mediated gene silencing. |
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|
Citations |
Western Blotting - Otsuka M et al. Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma. PLoS One. 6, e24359 (2011)(PMID:21935400)
- Fukao A et al. MicroRNAs Trigger Dissociation of eIF4AI and eIF4AII from Target mRNAs in Humans. Mol Cell 56, 79-89 (2014)(PMID:25280105)
- Yamagishi M et al. Coordinated loss of microRNA group causes defenseless signaling in malignant lymphoma. Sci Rep. 5, 17868 (2015)(PMID:26639163)
- Kami D et al. The DEAD-box RNA-binding protein DDX6 regulates parental RNA decay for cellular reprogramming to pluripotency. PLoS One. 13, e0203708 (2018)(PMID:30273347)
Immunocytochemistry - Otsuka M et al. Receptor for activated protein kinase C: requirement for efficient microRNA function and reduced expression in hepatocellular carcinoma. PLoS One. 6, e24359 (2011)(PMID:21935400)
- Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
RNP Immunoprecipitation - Yamagishi M et al. Coordinated loss of microRNA group causes defenseless signaling in malignant lymphoma. Sci Rep. 5, 17868 (2015)(PMID:26639163)
Other(eCLIP) - Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 61, 903-13 (2016)(PMID:26990993)
- Van Nostrand EL et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods. 13, 508-14 (2016)(PMID:27018577)
|
Product category |
- Research area
- RNA-RNP Network
Exosomes - Brands
- RiboCluster Profiler™
|